1. pT3TS-Tol2

   Plasmid

   #31831

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Lac

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pcDNA3.1-Flag-NPM1c

   Plasmid

   #131813

   Purpose

   Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   This is the cytoplasmic mutant causing AML, where…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAcGFP-Hyg-CTCF-N1

   Plasmid

   #131799

   Purpose

   Expresses human CTCF-GFP in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFlare9A-CD45i4e5i5mutCBS

   Plasmid

   #131835

   Purpose

   A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   PTPRC (PTPRC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial intron 4 full exon 5 and partial intron 5…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHcRed-NPMMLF1nes-C1

   Plasmid

   #131849

   Purpose

   Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   This is a control deletion contruct of the cytop…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAcGFP-Hyg-CTCFdZnF-N1

   Plasmid

   #131803

   Purpose

   Expresses the N and C terminus of human CTCF-GFP with the zinc finger domain removed in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-1731

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAcGFP-Hyg-CTCF-ZnF-N1

   Plasmid

   #131802

   Purpose

   Expresses the [Zinc finger domain of human CTCF (a.a. 265-603)]-GFP in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-1731

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAcGFP-Hyg-CTCF-Cterm-N1

   Plasmid

   #131801

   Purpose

   Expresses the [C-terminus of human CTCF (a.a. 577-728)]-GFP in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-1731

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHcRed-NPM1cdN243-C1

   Plasmid

   #131838

   Purpose

   Expresses the last 50 amino acids of HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   This is a deletion contruct of the cytoplasmic m…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHcRed-NPM1cOligodN243-C1

   Plasmid

   #131847

   Purpose

   Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   This is a deletion contruct of the cytoplasmic m…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHcRed-NPM1cNLSdN243-C1

    Plasmid

    #131848

    Purpose

    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is a deletion contruct of the cytoplasmic m…

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmBanana-Hyg-CTCF-CtermNES-N1

    Plasmid

    #131851

    Purpose

    Expresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cells

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    mBanana

    Expression

    Mammalian

    Mutation

    the C-terminus of CTCF is linked to HIV-Rev prote…

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmBanana-Hyg-CTCF-NtermNES-N1

    Plasmid

    #131850

    Purpose

    Expresses the [N-terminus of human CTCF (a.a. 2-265)]-mBanana with a nuclear export signal in mammalian cells

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    mBanana

    Expression

    Mammalian

    Mutation

    deleted amino acids 796-2181 (the N-terminus is l…

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAcGFP-Hyg-CTCF-Nterm-N1

    Plasmid

    #131800

    Purpose

    Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 796-2181 (the N-terminus is l…

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge