NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia.
Wang AJ, Han Y, Jia N, Chen P, Minden MD
Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. PubMed Article
Wang AJ, Han Y, Jia N, Chen P, Minden MD
Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131799 | pAcGFP-Hyg-CTCF-N1 | Expresses human CTCF-GFP in mammalian cells through transient transfection |
| 131800 | pAcGFP-Hyg-CTCF-Nterm-N1 | Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells |
| 131801 | pAcGFP-Hyg-CTCF-Cterm-N1 | Expresses the [C-terminus of human CTCF (a.a. 577-728)]-GFP in mammalian cells |
| 131802 | pAcGFP-Hyg-CTCF-ZnF-N1 | Expresses the [Zinc finger domain of human CTCF (a.a. 265-603)]-GFP in mammalian cells |
| 131803 | pAcGFP-Hyg-CTCFdZnF-N1 | Expresses the N and C terminus of human CTCF-GFP with the zinc finger domain removed in mammalian cells |
| 131810 | pcDNA3.1-Flag-NPM1wt | Expresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection |
| 131813 | pcDNA3.1-Flag-NPM1c | Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection |
| 131818 | pHcRed-NPM1wt-C1 | Expresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfection |
| 131821 | pHcRed-NPM1c-C1 | Expresses HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection |
| 131822 | p5xUAS-Flag-NPM1wt | 4-hydroxytamoxifen inducible vector expressing Flag-NPM1wildtype (human) in mammalian cells through transduction |
| 131823 | p5xUAS-Flag-NPM1c | 4-hydroxytamoxifen inducible vector expressing Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transduction |
| 131824 | pLVX-NPM1wt-IRES-mCherry | Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction |
| 131825 | pLVX-NPM1c-IRES-mCherry | Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction |
| 131834 | pFlare9A-CD45i4e5i5 | A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a CTCF binding site. |
| 131835 | pFlare9A-CD45i4e5i5mutCBS | A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded. |
| 131838 | pHcRed-NPM1cdN243-C1 | Expresses the last 50 amino acids of HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection |
| 131847 | pHcRed-NPM1cOligodN243-C1 | Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection |
| 131848 | pHcRed-NPM1cNLSdN243-C1 | Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection |
| 131849 | pHcRed-NPMMLF1nes-C1 | Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection |
| 131850 | pmBanana-Hyg-CTCF-NtermNES-N1 | Expresses the [N-terminus of human CTCF (a.a. 2-265)]-mBanana with a nuclear export signal in mammalian cells |
| 131851 | pmBanana-Hyg-CTCF-CtermNES-N1 | Expresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cells |
| 131852 | pHyg-N1 | This is the empty vector control for the mBanana competition experiment |