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pAcGFP-Hyg-CTCF-N1
(Plasmid #131799)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131799 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 131799-D50 50 μg of DNA in Tris buffer $495
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DNA 131799-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pAcGFP-Hyg-N1
  • Backbone size w/o insert (bp) 5791
  • Total vector size (bp) 7936
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTCF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2181
  • Entrez Gene
    CTCF (a.k.a. CFAP108, FAP108, MRD21)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GGAG GTCTATATAA GCAGAGCTG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Elena Pugacheva

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains an IS4-like element between the AmpR promoter and AmpR resistance gene. This discrepancy is not known to affect plasmid function as plasmid was able to grow in Ampicillin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGFP-Hyg-CTCF-N1 was a gift from Mark Minden (Addgene plasmid # 131799 ; http://n2t.net/addgene:131799 ; RRID:Addgene_131799)

  • For your References section:

    NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Wang AJ, Han Y, Jia N, Chen P, Minden MD. Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. 10.1038/s41375-019-0681-8 PubMed 31831844
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