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Plasmid#43219DepositorInsertII-AAAA
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
I-AAAA
Plasmid#42963DepositorInsertI-AAAA
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
RFP-PPPPPPAAAA-gs
Plasmid#228092PurposeMammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cellsDepositorInsertmCit, PPPPPPAAAA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
III-AAAA
Plasmid#43475DepositorInsertIII-AAAA
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alani…PromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT7 LDIG69AAAA
Plasmid#61560PurposeHs GST-PRMT7 LDIG(69-72)AAAA AdoMet binding deficient mutantDepositorInsertPRMT7 (PRMT7 Human)
UseTagsGSTExpressionBacterialMutationLDIG 69-72 AAAAPromoterAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 AAAA
Plasmid#163579Purposeexpress Dcp2 300 AAAA at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); mutati…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(151-154AAAA)
Plasmid#166131PurposeExpression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.DepositorInsertT1head1-405(151-154AAAA) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationaa1-405 only. Contains 4 amino acid substitutions…PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD173 ZF43(AAAA)i
Plasmid#138833PurposeConstitutive expression of ZF43(AAAA)i under the CMV promoter.DepositorInsertZF43(AAAA)i
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6A, R15A, R43A, R71APromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 AAAA
Plasmid#87600PurposeEncodes Prp5 AAAA for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD136A, P137A, L138A, D139APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme N40-[AAAA] WT*
Plasmid#18299DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationN40-[AAAA] WT*PromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme S44-[AAAA] WT*
Plasmid#18320DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationS44-[AAAA] WT*PromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme K48-[AAAA] WT*
Plasmid#18343DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationK48-[AAAA] WT*PromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-YFWFtoAAAA_AH
Plasmid#148913PurposeInsect Expression of DmGIGYF-YFWFtoAAAADepositorInsertDmGIGYF-YFWFtoAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF-YFWFtoAAAA_AH
Plasmid#148922PurposeInsect Expression of DmGIGYF-YFWFtoAAAADepositorInsertDmGIGYF-YFWFtoAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-CLLKtoAAAA_AH
Plasmid#148924PurposeInsect Expression of DmMe31B-CLLKtoAAAADepositorInsertDmMe31B-CLLKtoAAAA (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-QHTKtoAAAA-dsRNAres_AC
Plasmid#148391PurposeInsect Expression of DmMe31B-QHTKtoAAAA-dsRNAresDepositorInsertDmMe31B-QHTKtoAAAA-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2d (FRRG/AAAA)-TS
Plasmid#190037PurposeExpression of wipi2d mutantDepositorInsertWIPI2d (WIPI2 Human)
UseTagsTEV-2xStrep tagExpressionMammalianMutation223FRRG to AAAAPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsRck-CLLKtoAAAA_AI
Plasmid#148966PurposeMammalian Expression of HsRck-CLLKtoAAAADepositorInsertHsRck-CLLKtoAAAA (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-CLLKtoAAAA_AI
Plasmid#148956PurposeMammalian Expression of HsRck-CLLKtoAAAADepositorInsertHsRck-CLLKtoAAAA (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only