We narrowed to 24 results for: aaas
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  TypeBlog Post...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans...even through all my roles and my current work as a AAAS Science and Technology Policy Fellow (STPF) at the...roles exist. Explore these resources compiled by the AAAS Science and Technology Policy Fellowship Program... to late-career backgrounds. In fact, many of my AAAS STPF cohort members came to DC to do either a sabbatical...Many thanks to our guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans...Administration. Steph Guerra, PhD, is currently a AAAS Science and Technology Policy Fellow at the Veterans...
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  Science Careers: Unruly Interests Feed Many PathsTypeBlog Post...American Association for the Advancement of Science (AAAS), we have all sorts of different editors. Do you...a short program to build skills and connections. AAAS offers a summer program that gives graduate students...radio, print journalism, and other media outlets (AAAS Mass Media Science & Engineering Fellows Program...
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  Science communication: 9 Strategies to Get Your Foot in the DoorTypeBlog Post...science communication societies like @BritSciAssoc, @AAAS, @Stempra and science blogs like @WIRED Science....communicators? Attend the annual flagship meetings of AAAS, WCSJ or ECSJ. These meeting are THE opportunity...here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship, which places science students...
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  Scientific Reproducibility - Focusing on Solutions at the Minisymposium on ReproducibilityTypeBlog Post...Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel, irreproducibility undermines...into practice! Additional Resources SciLine at AAAS - A service connecting journalists and scientific...
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  10 Ways to Share Your Science!TypeBlog Post...to get serious about science writing, look into a AAAS Mass Media Fellowship or maybe an internship at ...
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  Savvy Advocates Needed to Navigate a Scientific Enterprise in FluxTypeBlog Post...PhD - Director, Early Career Scientist Segment at AAAS Marnie Gelbart, PhD - Director of Programs at Personal...
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  Components of CRISPR/Cas9TypeBlog Post...ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC The synthetic guide RNA or gRNA (sometimes ...
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  Plasmids 101: Terminators and PolyA signalsTypeBlog Post...hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation and termination...Position-dependent sequence elements downstream of AAUAAA are required for efficient rabbit beta-globin mRNA...
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  Transgenic Organisms, Cas9 Gene Drives, and Appropriate SafeguardsTypeBlog Post...homozygous mutations. Science aaa5945 (2015). doi:10.1126/science.aaa5945. PubMed. DiCarlo, J. E., Chavez...
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  The PAM Requirement and Expanding CRISPR Beyond SpCas9TypeBlog Post...thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional Cas9 species PAM sequence ...
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  CRISPR/Cas9 FAQs Answered!TypeBlog Post...order is:5'-CACCGN...N-3'3'-CN...NCAAA-5'You need to add the CACC and AAAC cloning overhang. Q3: When designing...
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  Validated gRNA SequencesTypeCollection...thaliana TTTGAAAGATGGAAGCGCGG cut S. pyogenes 26479191 Kim BRI1 A. thaliana TGAAACTAAACTGGTCCACA cut S. ...Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG 66989 cut S. pyogenes 26028531 Huangfu OCT4... pyogenes 26541286 Voytas APC H. sapiens GAAGCGGGCAAAGGGGCGAC 58780 cut/nick S. pyogenes 24954249 Yamamoto...pyogenes 25619936 Sato ASCL1 H. sapiens TGGGGCTGGGTGTCCCATTGAAA 64129 activate S. pyogenes 25619936 Sato...pyogenes 24967838 Mashimo ATF1 H. sapiens TAGGAATCAAACACTTTTATTGG 64690 tag S. pyogenes 26355004 Mendenhall...pyogenes 24954249 Yamamoto BLIMP1 H. sapiens CGGATGGGGTAAACGACCCG 59724 cut S. pyogenes 25543152 Hanna BT1754...BT1754 B. thetaiotaomicron GAAAATGGGGTGTATCCTGC 68892 interfere S. pyogenes 26918244 Lu BT1854 B. thetaiotaomicron...
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  Protocol - pLKO.1 – TRC Cloning VectorTypeProtocol...antisense—TTTTTG 3’ Reverse oligo: 5’ AATTCAAAAA—21bp sense—CTCGAG—21bp antisense 3’ For example...GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse oligo: 5’ AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT...
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  Zinc Finger Consortium: Zinc Finger ArraysTypeCollection...gCCCCTCAGCccagatgGGAGGAGATg dpf2 OZ531 and OZ532 OPEN OPEN cTGCTACTTCtggatGGAGAAACGa pitx2 OZ533 and OZ534 OPEN OPEN cCGCTTCAGCggtctGTGGACTCGa...Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc...Gene (sbds) OZ545 and OZ546 OPEN OPEN tGTTGCCGTCgtgagGATGAAGAAa CACNA1D (2of2) or cav1.3b OZ547 and OZ548... H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa GUSB OZ551 and OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa...tTACCTCCACGATCTTGCTGATGGAg dio2 OZ569 and OZ570 CODA OPEN aAGCTGCTCCAAACTCTAAGGTGGTg im:7163548 OZ571 and OZ572 CODA CODA ...001003579.1) OZ575 and OZ576 CODA OPEN cAACCTCAGCATAAAGCTGGAGGAg psen2 OZ577 and OZ578 CODA OPEN cGGCATCTCCAGAGCCCGAGGCGGACc...A4QNT7_DANRE) OZ585 and OZ586 CODA CODA cTGCACCTTCACAAAAGGCCGCTGGCt LOC566040 OZ587 and OZ588 OPEN OPEN cAGCACCAGCCAGCCCAGAGTTGGGGt...
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  Pairing CombiGEM and CRISPR for Combinatorial Genetic ScreeningTypeBlog Post...; Reverse oligo: 5’- AATTGXXXXXXXXGAATAGGTCTTCTCGAAGACCCAAACNNNNNNNNNNNNNNNNNNNC -3’, where NNN and XXX...
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  Cre-Lox and Other Site-Specific RecombinasesTypeCollection... lox511, loxN, lox66/lox71 Dre rox TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer...site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC 13 bp inverted repeats + 8... F3, F5, FRT14, FRT15 minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and...
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  Molecular Biology ReferenceTypeGuide... Leu L UUA, UUG, CUU, CUC, CUA, CUG Lysine Lys K AAA, AAG Methionine Met M AUG Phenylalanine Phe F UUU...PKKKRKV or PKKKRKVG Protein C EDQVDPRLIDGK S Tag KETAAAKFERQHMDS SB1 PRPSNKRLQQ Additional Resources Molecular...
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  Plasmids 101: Protein tagsTypeBlog Post...small size, rarely affects function S-tag KETAAAKFERQHMDS 1.8 Solubility and Affinity Abundance of charged...
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  Neurodegeneration Plasmid CollectionTypeCollection...GIGYF2 GST tac Parkinson's Sachdev Sidhu 109964 pHH0103_VCP_AAA VCP GST tac ALS Sachdev Sidhu 110028 pHH0103...Elisa Izaurralde 148521 pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD EIF4G GFP CMV Parkinson's Elisa Izaurralde 148522...Izaurralde 148523 pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD EIF4G GFP CMV Parkinson's Elisa Izaurralde 148524...Izaurralde 148580 pT7-EGFP-C1-HsGIGYF2_1-180-YYMLtoAAAA_AE GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...Elisa Izaurralde 148691 pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AF GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...Izaurralde 148950 pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...
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  28 Hot Plasmid Technologies from 2015TypeBlog Post...2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380 MuLE vectors for customized lentivirus production...