We narrowed to 26 results for: aaas
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TypeBlog Post...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans...even through all my roles and my current work as a AAAS Science and Technology Policy Fellow (STPF) at the...roles exist. Explore these resources compiled by the AAAS Science and Technology Policy Fellowship Program... to late-career backgrounds. In fact, many of my AAAS STPF cohort members came to DC to do either a sabbatical...Many thanks to our guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans...Administration. Steph Guerra, PhD, is currently a AAAS Science and Technology Policy Fellow at the Veterans...
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Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...American Association for the Advancement of Science (AAAS), we have all sorts of different editors. Do you...a short program to build skills and connections. AAAS offers a summer program that gives graduate students...radio, print journalism, and other media outlets (AAAS Mass Media Science & Engineering Fellows Program... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...science communication societies like @BritSciAssoc, @AAAS, @Stempra and science blogs like @WIRED Science....communicators? Attend the annual flagship meetings of AAAS, WCSJ or ECSJ. These meeting are THE opportunity...here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship, which places science students... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel, irreproducibility undermines...into practice! Additional Resources SciLine at AAAS - A service connecting journalists and scientific... -
10 Ways to Share Your Science!
TypeBlog Post...to get serious about science writing, look into a AAAS Mass Media Fellowship or maybe an internship at ... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...PhD - Director, Early Career Scientist Segment at AAAS Marnie Gelbart, PhD - Director of Programs at Personal... -
Components of CRISPR/Cas9
TypeBlog Post...ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC The synthetic guide RNA or gRNA (sometimes ... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation and termination...Position-dependent sequence elements downstream of AAUAAA are required for efficient rabbit beta-globin mRNA... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...homozygous mutations. Science aaa5945 (2015). doi:10.1126/science.aaa5945. PubMed. DiCarlo, J. E., Chavez... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional Cas9 species PAM sequence ... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...order is:5'-CACCGN...N-3'3'-CN...NCAAA-5'You need to add the CACC and AAAC cloning overhang. Q3: When designing... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...antisense—TTTTTG 3’ Reverse oligo: 5’ AATTCAAAAA—21bp sense—CTCGAG—21bp antisense 3’ For example...GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse oligo: 5’ AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...ahcyl1 TAL3018 & TAL3019 TGACAGACTGTGGCGAGGatgccaagctggaggcGACGAAACAGGCCAGCAA amh TAL3210 & TAL3211 TCCAGGCAAGATTTGGGCtgatgctgatgatgacCGTGGCGATTGGGTCGTA... apoa4 TAL3022 & TAL3025 TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA apoa4-like TAL3023 & TAL3024...TAL3024 TGAAGGTTCTTGTGGTGCtcacacttgctgtggtTACAGGTAAGAAACTAAA apoeb TAL3402 & TAL3403 TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA... asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA atf6 TAL3406 & TAL3407 TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA... TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA atg5 TAL3218 & TAL3219 TAAAGTCAAGAAACATTTtctcaaagtcatgaagGCAGAGGATGTGGAGGAA...TCAGCTCCCCGGAGAGGCaccgcactgctccagggTTGGATGATGGACATGGA bsx TAL3034 & TAL3035 TCTCAGACTCACAGTTATccggactcgagaaaagGTTCGAGATCCAGAGATA btr30 TAL3036 & TAL3037 ...casp8ap2 TAL3038 & TAL3039 TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA Ccl2 TAL3222 & TAL3223 TTTTCGTTTTCCACTATAaagatcccagttaagcAGGTTCAGAGTTATCATA... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...; Reverse oligo: 5’- AATTGXXXXXXXXGAATAGGTCTTCTCGAAGACCCAAACNNNNNNNNNNNNNNNNNNNC -3’, where NNN and XXX... -
Validated gRNA Sequences
TypeCollection...thaliana TTTGAAAGATGGAAGCGCGG cut S. pyogenes 26479191 Kim BRI1 A. thaliana TGAAACTAAACTGGTCCACA cut S. ...Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG 66989 cut S. pyogenes 26028531 Huangfu OCT4... pyogenes 26541286 Voytas APC H. sapiens GAAGCGGGCAAAGGGGCGAC 58780 cut/nick S. pyogenes 24954249 Yamamoto...pyogenes 25619936 Sato ASCL1 H. sapiens TGGGGCTGGGTGTCCCATTGAAA 64129 activate S. pyogenes 25619936 Sato...pyogenes 24967838 Mashimo ATF1 H. sapiens TAGGAATCAAACACTTTTATTGG 64690 tag S. pyogenes 26355004 Mendenhall...pyogenes 24954249 Yamamoto BLIMP1 H. sapiens CGGATGGGGTAAACGACCCG 59724 cut S. pyogenes 25543152 Hanna BT1754...BT1754 B. thetaiotaomicron GAAAATGGGGTGTATCCTGC 68892 interfere S. pyogenes 26918244 Lu BT1854 B. thetaiotaomicron... -
Plasmids 101: Protein tags
TypeBlog Post...small size, rarely affects function S-tag KETAAAKFERQHMDS 1.8 Solubility and Affinity Abundance of charged... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...gCCCCTCAGCccagatgGGAGGAGATg dpf2 OZ531 and OZ532 OPEN OPEN cTGCTACTTCtggatGGAGAAACGa pitx2 OZ533 and OZ534 OPEN OPEN cCGCTTCAGCggtctGTGGACTCGa...Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc...Gene (sbds) OZ545 and OZ546 OPEN OPEN tGTTGCCGTCgtgagGATGAAGAAa CACNA1D (2of2) or cav1.3b OZ547 and OZ548... H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa GUSB OZ551 and OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa...tTACCTCCACGATCTTGCTGATGGAg dio2 OZ569 and OZ570 CODA OPEN aAGCTGCTCCAAACTCTAAGGTGGTg im:7163548 OZ571 and OZ572 CODA CODA ...001003579.1) OZ575 and OZ576 CODA OPEN cAACCTCAGCATAAAGCTGGAGGAg psen2 OZ577 and OZ578 CODA OPEN cGGCATCTCCAGAGCCCGAGGCGGACc...A4QNT7_DANRE) OZ585 and OZ586 CODA CODA cTGCACCTTCACAAAAGGCCGCTGGCt LOC566040 OZ587 and OZ588 OPEN OPEN cAGCACCAGCCAGCCCAGAGTTGGGGt... -
Cre-lox system
TypeCollection...Capecchi 137860 pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA) iCre-ERT2 (PAAA N-terminus) - Tamoxifen inducible CAG Mammalian...Capecchi 137861 pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAAA) iCre-ERT2 (PAAAA N-terminus) - Tamoxifen inducible CAG Mammalian... -
Molecular Biology Reference
TypeGuide... Leu L UUA, UUG, CUU, CUC, CUA, CUG Lysine Lys K AAA, AAG Methionine Met M AUG Phenylalanine Phe F UUU...PKKKRKV or PKKKRKVG Protein C EDQVDPRLIDGK S Tag KETAAAKFERQHMDS SB1 PRPSNKRLQQ Webpage and Blog References ... -
Neurodegeneration Plasmid Collection
TypeCollection...GIGYF2 GST tac Parkinson's Sachdev Sidhu 109964 pHH0103_VCP_AAA VCP GST tac ALS Sachdev Sidhu 110028 pHH0103...Elisa Izaurralde 148521 pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD EIF4G GFP CMV Parkinson's Elisa Izaurralde 148522...Izaurralde 148523 pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD EIF4G GFP CMV Parkinson's Elisa Izaurralde 148524...Izaurralde 148580 pT7-EGFP-C1-HsGIGYF2_1-180-YYMLtoAAAA_AE GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...Elisa Izaurralde 148691 pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AF GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...Izaurralde 148950 pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI GIGYF2 GFP CMV Parkinson's Elisa Izaurralde ...