We narrowed to 14,587 results for: pET

1. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

   Plasmid

   #226349

   Purpose

   Expression of Titin V15P substrate with GS tail

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

   Plasmid

   #169135

   Purpose

   E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Coh7
   

   Use

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

   Plasmid

   #218973

   Purpose

   Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant SRSF1 binding motif (AGTCTCCT)

   Promoter

   RSV LTR

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

   Plasmid

   #208626

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 191~307, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

   Plasmid

   #208627

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 299~398, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

   Plasmid

   #208629

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 212~285, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETM-60-NcQDE2 _506-938-del787-838-GGSG_O

   Plasmid

   #147085

   Purpose

   Bacterial Expression of NcQde-2_506-938-del787-838

   Depositor

   Elisa Izaurralde

   Insert

   NcQde-2_506-938-del787-838
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETM-60-NcQDE2 _506-938-del787-838-GSG_O

   Plasmid

   #147086

   Purpose

   Bacterial Expression of NcQde-2_506-938-del787-838

   Depositor

   Elisa Izaurralde

   Insert

   NcQde-2_506-938-del787-838
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-15b(+) Mortierella verticillate (delta prenylation motif)

   Plasmid

   #170788

   Purpose

   Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)

   Depositor

   Kalle Gehring

   Insert

   Mortierella verticillate PRL
   

   Use

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   Deleted nucleotides coding for 4 C-terminal amino…

   Promoter

   AmpR, lacI

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid

    #170789

    Purpose

    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Toxoplasma gondii PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lacI

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Plasmid

    #166422

    Purpose

    Express pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Plasmodium falciparum PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)

    Plasmid

    #166423

    Purpose

    Express pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Pythium oligandrum PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)

    Plasmid

    #166424

    Purpose

    Express pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Reticulomyxa filosa PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

    Plasmid

    #166425

    Purpose

    Express pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Sphaeforma arctica PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

    Plasmid

    #166415

    Purpose

    Express pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Acytostelium subglobosum PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)

    Plasmid

    #166416

    Purpose

    Express pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    caenorhabditis elegans PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-15b(+) Danio rerio PRL (Δprenylation motif)

    Plasmid

    #166417

    Purpose

    Express pET-15b(+) Danio rerio PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Danio rerio PRL (ptp4a3a Zebrafish)
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

    Plasmid

    #166418

    Purpose

    Express pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Drosophila melanogaster PRL (Prl Fly)
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)

    Plasmid

    #166419

    Purpose

    Express pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Galdieria sulphuraria PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)

    Plasmid

    #166420

    Purpose

    Express pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Monosiga brevicollis PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge