We narrowed to 16,981 results for: tes
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Plasmid#174150PurposeLentiviral vector expressing Cas9 and a sgRNA against the human RB1 geneDepositorInsertRB1 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-Tpt1
Plasmid#42571DepositorInsertTpt1
Tags6xHistidineExpressionBacterialPromoterT7 RNA PolmyeraseAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGZ110 (pFA6a-3FLAG-MNase-TRP1)
Plasmid#70233PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tagDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGCS-C3m(3xFLAG)
Plasmid#85726PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTags3xFLAGAvailable SinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
5.2 kB VEGF-luc
Plasmid#27987DepositorInsert5.2 kB VEGF
UseLuciferaseAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1a-mCherryBCL11B-W
Plasmid#208758PurposeLentiviral vector expressing mCherry fused with BCL11B cDNADepositorInsertBCL11B (BCL11B Human)
UseLentiviralAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLH
Plasmid#201671PurposeExpression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cellsDepositorInsertPolymerase eta (POLH Human)
TagsHAExpressionMammalianMutationCoding sequence has been optimised for expression…PromoterCMVAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CXCR4
Plasmid#98944PurposeMammalian expression plasmid for untagged human CXCR4DepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S
Plasmid#62458Purposeexpresses his-tagged C151S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
TagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 151 of Keap1 to serinePromoterCMVAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSara
Plasmid#60720PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFDepositorInsertsN Terminal fragment of T7 RNAP split betwen 179-180
N Terminal fragment of T7 RNAP split betwen 179-180
ExpressionBacterialMutationT7 RNAP split between positions 179-180 and wt T7…PromoterPBADAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
p817-mgammaFcry-EGFP
Plasmid#23156DepositorInsertmouse gammaF crystallin promoter:EGFP (eGFP mouse gammaF promoter:EGFP, Mouse)
UseMulticloning site flanked by i-scei sitesMutationmouse gammaF crystallin promoter (-225 - +73) pre…Available SinceApril 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
Tol2-UAS-Dendra2
Plasmid#97145PurposeFor Gal4/UAS expression systemDepositorInsertDendra2
UseZebrafishAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pReadthroughUAA
Plasmid#11512DepositorInsertFirefly luciferase, UAA stop codon, Renilla luciferase, and 3' UTR short RNA binding sites
UseLuciferaseAvailable SinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pReadthroughUGA
Plasmid#11513DepositorInsertFirefly luciferase, UAA stop codon, Renilla luciferase, and 3' UTR short RNA binding sites
UseLuciferaseAvailable SinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
NeonGreen-C1-ORP5
Plasmid#220071Purposeoverexpresses ORP5 with a neonGreen fluorophoreDepositorInsertOSBPL5 (OSBPL5 Human)
ExpressionMammalianAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDNp
Plasmid#162451PurposePositive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.DepositorInsertAga2p-eUnaG2
UseShuttle vector bacteria/yeastTagsHA and mycPromoterGAL1Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHKMC1: Empty sgRNA for Cloning
Plasmid#67720PurposeEmpty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.DepositorTypeEmpty backboneExpressionWormPromoterPU6Available SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mStrawberry_Atg4BC74A
Plasmid#161735PurposeDoxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagyDepositorInsertAtg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsmStrawberryExpressionMammalianMutation220-222 TGC (Cys) is changed to GCA (Ala)Available SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-YFP
Plasmid#55782PurposeThis G protein alpha-q construct contains internal insertions of YFP and the EE epitope.DepositorInsertalpha-q EE YFP (Gnaq Mouse)
TagsThe EE epitope was introduced into the alpha-q se…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28c(+)-YTHDF3
Plasmid#102276PurposeFor bacterial expression of His-tagged human YTHDF3DepositorAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only