We narrowed to 4,705 results for: golden gate
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Plasmid#174561Purposemitochondrial-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vectorDepositorInsertDdCBE G1333-C
TagsFLAGExpressionPlantPromoterPcUbiAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1333-N
Plasmid#174560Purposemitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vectorDepositorInsertDdCBE G1333-N
TagsHAExpressionPlantPromoterPcUbiAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHW110031
Plasmid#222437PurposeLevel 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.DepositorInsertCBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW100002
Plasmid#222440PurposeLevel 0, CDS module (AATG-GCTT) containing rice OsL5.DepositorInsertOsL5 (Oryza sativa)
UseSynthetic BiologyExpressionPlantMutationBsaI/BbsI sites removed by point-mutationAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS1528
Plasmid#205982PurposeU6-MWEE01000013 Cas5-HNH DR-BpiI-DRDepositorInsertCas5-HNH DR-BpiI-DR
ExpressionMammalianPromoterU6Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTK403
Plasmid#110599PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
EC71167
Plasmid#154066PurposeLevel 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFPDepositorInsertZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
UseSynthetic BiologyMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterZea mays Ubiquitin PromoterAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-LacZa-T7
Plasmid#173224PurposepFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systemsDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK401
Plasmid#110597PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPS0384
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0385
Plasmid#133235PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0386
Plasmid#133236PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0383
Plasmid#133233PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1333-N
Plasmid#174556PurposeChloroplast-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vectorDepositorInsertDdCBE G1333-N
TagsHAExpressionPlantPromoterPcUbiAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1333-C
Plasmid#174557PurposeChloroplast-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vectorDepositorInsertDdCBE G1333-C
TagsFLAGExpressionPlantPromoterPcUbiAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2
Plasmid#182354PurposeNuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2
Plasmid#182356PurposeNuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-3
Plasmid#126058PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only