We narrowed to 5,502 results for: IRES

Showing: 4141 - 4160 of 5502 results

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pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN

Plasmid

#220128

Purpose

Expresses TadA8e and Sauri cas9N by MYL2 promoter, and add an HA tag fused to TadA

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauri Cas9N, inteinN

Use

AAV

Tags

HA tag

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA335

Plasmid

#216069

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; HygroR_v1.1; Stop

Use

Fragment

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AA333

Plasmid

#216067

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; PuroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; PuroR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA334

Plasmid

#216068

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; BlastR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; BlastR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Sap30bp-exon minimal CMV pCDNA5

Plasmid

#212084

Purpose

LR vector for integration of Sap30bp-exon_siResist into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Sap30bp-exon_siResist (Sap30bp Mouse)

Expression

Mammalian

Mutation

TCTAACCATCTGCAGGACA

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

Plasmid

#174897

Purpose

ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sf GFP

Expression

Mammalian

Mutation

150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

Plasmid

#174898

Purpose

opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TGA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pWalium Htt19Q-mCherry

Plasmid

#201248

Purpose

allows the expression of the insert in Drosophila melanogaster

Depositor

Pedro Domingos

Article

Branco-Santos et al Hum Mol Genet. 2017 Oct 1;26(19):3763-37

Insert

Huntingtin (HTT Human)

Expression

Insect

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-3-GST-DmGW182_1-593_F

Plasmid

#146271

Purpose

Bacterial Expression of DmGW182_1-593

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmGW182_1-593 (gw Fly)

Expression

Bacterial

Mutation

one non silent mutation T1772A (L591Q) compared t…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ZNF267

Plasmid

#178452

Purpose

doxycycline-inducible expression of Human ZNF267 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

ZNF267 (ZNF267 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-PLEK

Plasmid

#178439

Purpose

doxycycline-inducible expression of Human PLEK in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

PLEK (PLEK Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-RB_B

Plasmid

#145977

Purpose

Insect Expression of DmAGO1-RB

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmAGO1-RB (AGO1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

Plasmid

#159664

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh3

Plasmid

#168987

Purpose

Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

Plasmid

#159663

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

Plasmid

#159662

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

Plasmid

#159656

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDK616

Plasmid

#134202

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

K223A K226A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pIW040

Plasmid

#134127

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

aa 132-255 R236A

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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