We narrowed to 5,125 results for: golden gate
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Plasmid#239503PurposeExpress GAL-inducible cytosolic sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeurDepositorInsertsfCherry3C(1-10)-dPTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1280
Plasmid#239539PurposeExpress CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeurDepositorInsertNcCDT1-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1025
Plasmid#239496PurposeExpress inactive peroxisomal RsAS as negative controlDepositorInsertUbi-Y-RsAS(H360E)-Venus-ePTS1
ExpressionYeastMutationH360EAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1279
Plasmid#239538PurposeExpress CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2DepositorInsertScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1281
Plasmid#239540PurposeExpress CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative controlDepositorInsertNcCDT1-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235301PurposeComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1176
Plasmid#229871Purposegene trap RMCE plasmid with GFP-C1-tbb-2 3'UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertGFP-C1-tbb-2 3'UTR::SEC
TagsGFP-CExpressionWormAvailable SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLP-PH16_RS09790-pcepA-himar-chlorR
Plasmid#238494PurposeLanding Pad with BenM/PH16_RS09790, pC194+pKan+ptetQ seed promoter, pcepA-himar transposaseDepositorTypeEmpty backboneExpressionBacterialPromoterBenM/PH16_RS09790Available SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLP-ParaBAD-pcepA-himar-chlorR
Plasmid#238491PurposeLanding Pad with AraC/ParaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposaseDepositorTypeEmpty backboneExpressionBacterialPromoterAraC/ParaBADAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLP-PH16_RS08125-pcepA-himar-chlorR
Plasmid#238493PurposeLanding Pad with NahR/PH16_RS08125, pC194+pKan+ptetQ seed promoter, pcepA-himar transposaseDepositorTypeEmpty backboneExpressionBacterialPromoterNahR/PH16_RS08125Available SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2(miR.FF4)-bGH
Plasmid#235284PurposeComMAND open-loop circuit regulating mRuby2 with miR-FF4 design 2DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH
Plasmid#235277PurposeComMAND closed-loop circuit regulating mRuby2 with miR-FF5DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF6x4-bGH
Plasmid#235293PurposeComMAND open-loop circuit regulating mRuby2 with 4 copies of microRNA target sitesDepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2(miR.FF5)-bGH
Plasmid#235278PurposeComMAND open-loop circuit regulating mRuby2 with miR-FF5 design 2DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mGL-bGH
Plasmid#235299PurposemGreenLantern expression to serve as a transfection marker, EF1a-drivenDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH
Plasmid#235282PurposeComMAND open-loop circuit regulating mRuby2 with miR-FF4DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
U6-miR.FF4
Plasmid#235256PurposeNon-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)DepositorInsertFF4 hairpin
UseSynthetic BiologyExpressionMammalianPromoterU6Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF5x1-mRuby2-miR.FF5-bGH
Plasmid#235281PurposeComMAND closed-loop circuit regulating mRuby2 with miR-FF5 design 3DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235309PurposeComMAND open-loop circuit regulating mRuby2, EFS-drivenDepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEFS (human EF1a short, intronless)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2-bGH
Plasmid#235300PurposeComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only