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LLP750_L2pV1_AND_B3RT_FRT_Rluc_1IB_lacZ

Plasmid

#192394

Purpose

To act as a 1-input state of the AND gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP751_L2pV1_AND_B3RT_FRT_Rluc_2IFB_lacZ

Plasmid

#192395

Purpose

To act as a 2-input state of the AND gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP728_L2_pV01_0I_FRT-TB-rLUC_TCTP-fLUC

Plasmid

#192371

Purpose

To test for the repressive potential of the Transcription Block (TB) with the FRT sites around it.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-TB-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP673_L2_pV01_1I_VloxP-OCS-Rluc_TCTP_VCre_lacZ

Plasmid

#192425

Purpose

To act as the on state for testing of the alt VCre recombinase.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::VloxP-OCS-VloxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP671_L2_pV01_1I_SloxM1-OCS-Rluc_TCTP_SCre_lacZ

Plasmid

#192423

Purpose

To act as the on state for testing of the alt SCre recombinase.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::SloxM1-OCS-SloxM1::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP670_L2_pV01_0I_SloxM1-OCS-Rluc_lacZ

Plasmid

#192422

Purpose

To act as the off state for testing of the alt SCre recombinase.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::SloxM1-OCS-SloxM1::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP672_L2_pV01_0I_VloxP-OCS-Rluc_lacZ

Plasmid

#192424

Purpose

To act as the off state for testing of the alt VCre recombinase.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::VloxP-OCS-VloxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP674_L2_pV01_0I_vox-OCS-Rluc_lacZ

Plasmid

#192426

Purpose

To act as the off state for testing of the alt Vika recombinase.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::vox-OCS-vox::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

Plasmid

#192375

Purpose

To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-35Ster-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

Plasmid

#192374

Purpose

To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCStrunc-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

Plasmid

#192385

Purpose

To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

Plasmid

#192386

Purpose

To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP739_L2_pV01_0I_B3RT-OCS-Rluc_lacZ

Plasmid

#192383

Purpose

To act as the off state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP761_L2pV1_OR-OCS_s35S-Cre

Plasmid

#192420

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

Plasmid

#192373

Purpose

To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-TB-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

Plasmid

#192376

Purpose

To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-uORF-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP760_L2pV1_OR-OCS_TCTP-Flp

Plasmid

#192419

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZ

Plasmid

#192384

Purpose

To act as the on state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

Plasmid

#192377

Purpose

To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

Plasmid

#192378

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge