Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,447 results for: nls

Clear keywords
Showing: 4361 - 4380 of 6447 results

  2. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

    Enlarge

  5. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

    Enlarge

  7. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid
    #110626
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110626-plasmid-map-sequence-id-208002

    Enlarge

  8. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

    Enlarge

  12. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Promoter
    pRhaBAD
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

    Enlarge

  14. pCMV_dCas9_VP64

    Plasmid
    #49015
    Purpose
    encodes human-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (human-codon-optimized)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49015-plasmid-map-sequence-id-79086

    Enlarge

  15. pCAG-hCas9D10A

    Plasmid
    #52101
    Purpose
    The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter
    Depositor
    Izuho Hatada
    Insert
    hCas9D10A
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CAG
    Available Since
    April 30, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 4361 - 4380 of 6447 results