We narrowed to 5,125 results for: golden gate
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Plasmid#228638PurposeBacterial expression of the Coxsackievirus 63 3C proteaseDepositorInsertCoxsackievirus 63 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag
Plasmid#228635PurposeBacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus A71 3C protease isoform A
TagsHis-AviExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag
Plasmid#228634PurposeBacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus Coxsackievirus A71 2A protease
TagsHis-AviExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Human Coxsackievirus A16 strain G10 2A protease
Plasmid#228632PurposeBacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)DepositorInsertEnterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGLU_10
Plasmid#225818PurposeTn libraries Himar variant TET backboneDepositorTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1387
Plasmid#229880PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTRDepositorInsertPmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
TagsGFP-C1ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1643
Plasmid#229876Purposegene trap RMCE plasmid with Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertTet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1223
Plasmid#229875Purposegene trap RMCE plasmid with LexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertLexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1222
Plasmid#229874Purposegene trap RMCE plasmid with QF2[QF(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertQF2[QF(DBD)-linker-QFAD]-act-4 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1221
Plasmid#229873Purposegene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertQF-act-4 3' UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1177
Plasmid#229872Purposegene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertTet-Off[TetR-QFAD]-act-4 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1134
Plasmid#229870Purposegene trap RMCE plasmid with cGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertcGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW622
Plasmid#229868PurposeEntry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)DepositorInsertreverse FRT3 (rFRT3)
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
dSpyCas9-E566-GS-LOV2-VPR
Plasmid#231576PurposePotent light-deactivated CRISPRaDepositorInsertdSpyCas9-VPR with an AsLOV2 insertion behind E566
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3
Plasmid#231568PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertAll-in-one LOOMINA construct
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_dCas9-AsLOV2x3-NLSx3
Plasmid#231567PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
cpLOV2-Gal4
Plasmid#231569PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertcpLOV2-Gal4
ExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Zdk3-VP64
Plasmid#231571PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VP64
ExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
p35S-FHA-AtAGO4_gDNA
Plasmid#216838PurposeN-term Flag-HA tagged Arabidopsis thaliana (Col-0) AGO4 gDNA under regulation of CaMV 35S promoterDepositorAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
p35S-FHA-SpAGO4a_gDNA
Plasmid#216841PurposeN-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a gDNA under regulation of CaMV 35S promoterDepositorInsertARGONAUTE4
TagsFlag-HAExpressionPlantPromoterCaMV 35SAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only