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pLEW100v5-PUR-VSG-228.500bp

Plasmid

#245110

Purpose

Inserts 500 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:500

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.400bp

Plasmid

#245111

Purpose

Inserts 400 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:400

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.300bp

Plasmid

#245112

Purpose

Inserts 300 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:300

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.200bp

Plasmid

#245113

Purpose

Inserts 200 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:200

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.100bp

Plasmid

#245114

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:100

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.100bp_offset

Plasmid

#245115

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer overlapping AnTat1.1 position 694, centered around common VSG-228 insertion

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:100_offset

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CAPS1-mKate2

Plasmid

#245360

Purpose

Mammalian expression of C-terminal mKate2-tagged rat CAPS1 wild type

Depositor

Thomas Martin

Insert

Cadps (Cadps Rat)

Tags

mKate2

Expression

Mammalian

Promoter

CAGGS

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB085

Plasmid

#239637

Purpose

Plant RNA Vision constructs with 164 bp gRNAs together with RFP input

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Inserts

sfGFPn_Y66-P1_loop_01-stem01-gRNA09
gRNA10-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB084

Plasmid

#239636

Purpose

Plant RNA Vision constructs with 123 bp gRNAs together with RFP input

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Inserts

sfGFPn_Y66-P1_loop_01-stem01-gRNA07
gRNA08-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB086

Plasmid

#239638

Purpose

Plant RNA Vision constructs with 325 bp gRNAs together with RFP input

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Inserts

sfGFPn_Y66-P1_loop_01-stem01-gRNA11
gRNA12-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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rsZACRO/pcDNA3

Plasmid

#236199

Purpose

Mammalian expression of rsZACRO, a positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Ozaki-Noma et al ACS Nano. 2025 Feb 25;19(7):7188-7201. d

Insert

rsZACRO

Expression

Mammalian

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmKate2Spc

Plasmid

#206813

Purpose

Template for hlpA-mkate2 amplification associated with spectinomycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mKate2

Mutation

3insG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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REV7 C-terminal sgRNA

Plasmid

#207095

Purpose

pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GCTCATAAAGGCAGCTGAGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-MannII-N-10

Plasmid

#231769

Purpose

Visualization of Golgi in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-MannII-N-10 (Man2a1 Mouse)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Keratin-N-17

Plasmid

#231767

Purpose

Visualization of Keratin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Keratin-N-17 (KRT18 Human)

Tags

mGold2s

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-MannII-N-10

Plasmid

#231777

Purpose

Visualization of Golgi in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-MannII-N-10 (Man2a1 Mouse)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Keratin-N-17

Plasmid

#231775

Purpose

Visualization of Keratin in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Keratin-N-17 (KRT18 Human)

Tags

mGold2s

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pME AmCyan (JDW 1151)

Plasmid

#224485

Purpose

Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

AmCyan

Use

Gateway Cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME V5 mScarlet-I (JDW 1323)

Plasmid

#224487

Purpose

Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway Cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME iCre-I (JDW 1203)

Plasmid

#224520

Purpose

A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

iCre-I

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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