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pROS_phleo-Tps1/Gsy2

Plasmid

#196612

Purpose

Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

TPS1, GSY2 (TPS1, GSY2 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pIFEX-A2aGH

Plasmid

#160542

Purpose

pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEFEX1-A2aGH

Plasmid

#160543

Purpose

pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCFEX1-A2aGH

Plasmid

#160546

Purpose

pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pZ_P-ICL1-LacI-Mit1AD

Plasmid

#126721

Purpose

LacI-Mit1AD on pPICZ B expression vector

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Expression

Yeast

Promoter

ICL1

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-ICL1-LacI-VP16

Plasmid

#126723

Purpose

LacI-VP16 on pPICZ B expression vector

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-VP16 activation domain coding sequence

Expression

Yeast

Promoter

ICL1

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-ICL1-LacI-Gal4AD

Plasmid

#126724

Purpose

LacI-Gal4AD on pPICZ B expression vector

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Gal4 activation domain coding sequence

Expression

Yeast

Promoter

ICL1

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.27

Plasmid

#99362

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 129 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-AtSDT (JBEI-11535)

Plasmid

#87934

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSDT

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

SDT (SDT Mustard Weed)
4CL5 (4CL5 Mustard Weed)

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGPD2

Plasmid

#43972

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

GPD

Available Since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pLS410

Plasmid

#239123

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSL-MS2-12X

Plasmid

#27119

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (12)

Expression

Bacterial

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pRS1244

Plasmid

#247644

Purpose

Inducible CRISPRi-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Article

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pRS1005

Plasmid

#247641

Purpose

Constitutive CRISPRa-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Article

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pRS1074

Plasmid

#247642

Purpose

Constitutive CRISPRi-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Article

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pRS1242

Plasmid

#247643

Purpose

Inducible CRISPRa-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Article

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pSL-MS2-6X

Plasmid

#27118

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (6)

Expression

Bacterial

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pLS416

Plasmid

#239127

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pIB2

Plasmid

#25451

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCR4-24XMS2SL-stable

Plasmid

#31865

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

24xMS2 Stem Loops - Stable

Available Since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

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