We narrowed to 14,607 results for: pET

1. TrpLE-M-nMSP1E3D1

   Plasmid

   #179949

   Purpose

   Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

   Depositor

   Kerney Glover

   Article

   Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

   Insert

   TrpLE-M-nMSP1E3D1
   

   Use

   Tags

   TrpLE

   Expression

   Bacterial

   Mutation

   Native Trp residues have been mutated to Phe resi…

   Promoter

   T7

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Twin-Strep(R)-Tev-GSGS-Ubiquitin

   Plasmid

   #186803

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   Twin-Strep(R)-Tev-GSGS-Ubiquitin
   

   Use

   Tags

   GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

   Expression

   Bacterial

   Mutation

   Strep tag Lysine's mutated to Arginine

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

   Plasmid

   #183069

   Purpose

   Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c_4
   

   Use

   CRISPR

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   Wild-type

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CL7-LbCas12aD156R

   Plasmid

   #164659

   Purpose

   Bacterial expression of mutated LbCas12aD156R using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a-D156R
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 156 to Arginine

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP2

   Plasmid

   #26282

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009 . 464():211-31.

   Insert

   MSP2
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of dimer of deletion mutant (1-43)…

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pSC-SmBiT

   Plasmid

   #207632

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-SmBiT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Danio rerio cryabb

   Plasmid

   #47665

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha B-crystallin (cryabb Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZF-BCB-PDZ

   Plasmid

   #127478

   Purpose

   coding sequence for synthetic zinc finger ZF-BCB-PDZ

   Depositor

   Sebastian Maerkl

   Article

   Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

   Insert

   ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
   

   Use

   Cell-free transcription–translation

   Tags

   alpha 1-syntrophin PDZ domain

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZF-ADD-L

   Plasmid

   #127477

   Purpose

   coding sequence for synthetic zinc finger ZF-ADD-L

   Depositor

   Sebastian Maerkl

   Article

   Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

   Insert

   ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
   

   Use

   Cell-free transcription–translation

   Tags

   PDZ cognate peptide ligand (VKESLV)

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. small ubiquitin-like modifier 3 (SUMO3)

    Plasmid

    #221053

    Purpose

    Expression of small ubiquitin-like modifier 3 (SUMO3) protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    SUMO 3 (SUMO3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpyCatcher-P-Domain

    Plasmid

    #201193

    Purpose

    Expresses furin P-domain genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-P-Domain
    

    Use

    Tags

    HisTag and SpyCatcher001

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM57

    Plasmid

    #227654

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, V80A

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM54

    Plasmid

    #227653

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, L132A

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM72

    Plasmid

    #227622

    Purpose

    pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    His-Prescission

    Expression

    Bacterial

    Mutation

    1-179 and aa 2-239, G77P

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM71

    Plasmid

    #227621

    Purpose

    pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    His-Prescission

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 24-160-GGSGSG-192-221

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. nUnaG-ST

    Plasmid

    #207630

    Purpose

    A plasmid encoding the N-terminal UnaG fragment fused to SpyTag.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    nUnaG-SpyTag-MBP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 1-84

    Promoter

    T7

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpyCatcher-DIIG

    Plasmid

    #201196

    Purpose

    Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-DIIG
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SpyCatcher-SWG

    Plasmid

    #201194

    Purpose

    Expresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-SWG
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SpyCatcher-PEED

    Plasmid

    #201195

    Purpose

    Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-PEED
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHIT348

    Plasmid

    #204515

    Purpose

    Bacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen

    Depositor

    Yuji Kohara

    Article

    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

    Insert

    C-terminal fragment of cec-5 (C. elegans)
    

    Use

    Tags

    6xHis, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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