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pscAAV-U6-gSCR-CBh-mCherry-SV40late

Plasmid

#249124

Purpose

expression of gSCR (scrambled, non-targeting gRNA) and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gSCR (Scr Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-EFS-CasRx-pA

Plasmid

#192488

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

Plasmid

#203451

Purpose

Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-non targeting (NT) sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Codon optimised using GenScript tool

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-VGAT sesRNA-2a-msFlag-2a-tTA2-WPRE

Plasmid

#239030

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse VGAT (Slc32a1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB

Plasmid

#223660

Purpose

Expresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shLacZ

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

Plasmid

#114374

Purpose

expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

Plasmid

#114378

Purpose

expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-KA2N-EGFP (NEWENTRY )

Use

AAV

Tags

EGFP and KA2N

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD

Plasmid

#114371

Purpose

expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-MBD (NEWENTRY )

Use

AAV

Tags

EYFP and MBD

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna2

Plasmid

#192796

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna2

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna6

Plasmid

#192797

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna6

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna6

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

Plasmid

#198514

Purpose

Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CaMKIIα minimal promotor (0.4kb)

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40

Plasmid

#124604

Purpose

Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Insert

Soma-localized C1V1(t/t)

Use

AAV

Tags

mRuby2

Expression

Mammalian

Promoter

Ef1a

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV TRE3G:RiboL1-jGCaMP8f

Plasmid

#239677

Purpose

Soma-targeted (ribosome tethered via RiboL1) jGCaMP8f expressed under a TRE3G (tet responsive element) promoter.

Depositor

Marianne Fyhn

Article

Otero-Coronel et al bioRxiv 2026.05.28.728595

Insert

jGCaMP8f

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

TRE3G

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV TRE3G:RiboL1-jGCaMP8m

Plasmid

#239678

Purpose

Soma-targeted (ribosome tethered via RiboL1) jGCaMP8m expressed under a TRE3G (tet responsive element) promoter.

Depositor

Marianne Fyhn

Article

Otero-Coronel et al bioRxiv 2026.05.28.728595

Insert

jGCaMP8m

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

TRE3G

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-hA53T

Plasmid

#253535

Purpose

overexpression of a human mutated form of aSynuclein (A53T)

Depositor

Won Do Heo

Article

Hyeon et al Nat Commun. 2025 Aug 21;16(1):7797. doi:

Insert

hSNCA (SNCA Human)

Use

AAV

Mutation

A53T

Promoter

hSyn1

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ExSYTE.H64A

Plasmid

#216262

Purpose

inactive ExSYTE.H64A (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE P2A EGFP - Rat DTE

Use

AAV

Mutation

H64A mutation in each oTetR

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChRmine-ST-P2A-H2B-mRuby3

Plasmid

#227779

Purpose

Cation channelrhodopsin ChRmine targeted to the neuronal soma and proximal dendrites in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

DIO-ChRmine-ST-P2A-H2B-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

Plasmid

#112212

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

N-terminus of dCas9 and DNMT3A (DNMT3A Human)

Use

AAV

Mutation

H840A,N863A

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-hsChRmine-oScarlet-Kv2.1-WPRE

Plasmid

#183527

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

H33R

Promoter

Ef1a

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENAAV-cTNT-actn2-BioID2-HA

Plasmid

#216831

Purpose

cardiomyocytes-specific plasmid, expressing the coding sequence of mouse Actn2, biotin ligase BioID2, and a HA tag.

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

BioID-HA (Actn2 Synthetic)

Use

AAV

Tags

HA

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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