We narrowed to 6,923 results for: pcdna3.1

Showing: 5021 - 5040 of 6923 results

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ATG9A-2STrep

Plasmid

#210359

Purpose

Expresses ATG9A fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Publication

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

ATG9A (ATG9A Human)

Tags

2Strep

Expression

Mammalian

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f F366H

Plasmid

#204141

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

Depositor

Katalin Torok

Publication

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Phenylalanine 366 changed to histidine

Promoter

CMV

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG--uMASS_LF

Plasmid

#209763

Purpose

Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

Depositor

Andre Berndt

Publication

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Expression

Mammalian

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry-SEC61B

Plasmid

#207671

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61B

Depositor

Christine Mayr

Publication

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

SEC61B (SEC61B Human)

Tags

MCP-mCherry

Expression

Mammalian

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.20_mCherry-MS2x4

Plasmid

#205535

Purpose

Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.21_mCherry-MS2x6

Plasmid

#205536

Purpose

Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 mCherry EF1a

Plasmid

#206225

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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α-catenin ECFP-YPET FRET ΔF-actin binding site

Plasmid

#198245

Purpose

Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.

Depositor

Deborah Leckband

Publication

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

α-catenin ECFP-YPET FRET sensor ΔF-actin binding site

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Deleted F-actin binding site on a-catenin (849-90…

Promoter

CMV

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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V5-bArr1

Plasmid

#201491

Purpose

Expression of the beta-arrestin1 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Publication

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr1 (ARRB1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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bArr2-ALFA

Plasmid

#201498

Purpose

Expression of the beta-arrestin2 containing a N-terminal ALFA tag.

Depositor

Patrick Giguère

Publication

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr2-ALFA (ARRB2 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ELMOD1-myc-His6

Plasmid

#201900

Purpose

Used for expression of human protein ELMOD1 in mammalian cells

Depositor

Richard Kahn

Insert

ELMOD1 (ELMOD1 Human)

Tags

myc, 6xHis

Expression

Mammalian

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-nsp7-FLAG

Plasmid

#201027

Purpose

Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Publication

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

8xHis and FLAG

Expression

Mammalian

Promoter

T7 promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

Plasmid

#188992

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Publication

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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GFPxm163

Plasmid

#193480

Purpose

Turn-off fluorescent sensor for chloride

Depositor

Sheel Dodani

Publication

Peng et al Chem Sci. 2022 Oct 6;13(43):12659-12672.

Insert

GFPxm163

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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ChlorON-2

Plasmid

#193478

Purpose

Turn-on fluorescent sensor for chloride

Depositor

Sheel Dodani

Publication

Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

Insert

ChlorON-2

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP10-14-SEPT7

Plasmid

#180341

Purpose

mammalian expression of human SEPT7 fused to GFP10

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID

Plasmid

#188653

Purpose

Expresses SaAID in mammalian cells

Depositor

Keiji Nishida

Publication

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nSaCas9-AID-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

pCMV

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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IRSp53/BAIAP2 scFv [L117/1]

Plasmid

#190506

Purpose

Mammalian Expression of IRSp53/BAIAP2 scFV. Derived from hybridoma L117/1.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IRSp53/BAIAP2 (Homo sapiens) recombinant scFV (BAIAP2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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GAD67 scFv [L127/8]

Plasmid

#190509

Purpose

Mammalian Expression of GAD67 scFV. Derived from hybridoma L127/8.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GAD67 (Homo sapiens) recombinant scFV (GAD1 Mouse)

Tags

Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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VGlut1 scFv [N28/9]

Plasmid

#190545

Purpose

Mammalian Expression of VGlut1 scFV. Derived from hybridoma N28/9.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VGlut1 (Rattus norvegicus) recombinant scFV (Slc17a7 Mouse)

Tags

HA, 6xHis, Flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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