We narrowed to 6,824 results for: pcDNA3.1+
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Plasmid#126458PurposeExpresses Cas9-15xFlag-TdT in mammalian cells.DepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only
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pTBL681 CHYRON4 integration construct
Plasmid#126449PurposeTo integrate the CHYRON4 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON4 hgRNA
pCMV-puro
ExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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alpha-actinin-mGarnet2
Plasmid#104316Purposecodes for a fusion of fluorescent protein mGarnet2 and human alpha-actin, visualizes human alpha-actininDepositorInsertalpha-actinin-mGarnet2
ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)-G610C
Plasmid#119841PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagsmAppleExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα2(I)
Plasmid#119836PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagsmCeruleanExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mVenus-proα2(I)
Plasmid#119838PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagsmVenusExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR146
Plasmid#106047PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR146; Opto-GPR146; E1)DepositorInsertOpto-GPR146 (GPR146 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR139
Plasmid#106044PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR139; Opto-GPR139; D10)DepositorInsertOpto-GPR139
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR37L1
Plasmid#106020PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37L1; Opto-GPR37L1; B10)DepositorInsertOpto-GPR37L1 (GPR37L1 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR18
Plasmid#106006PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR18; Opto-GPR18; A8)DepositorInsertOpto-GPR18 (GPR18 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR19
Plasmid#106007PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)DepositorInsertOpto-GPR19 (GPR19 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR27
Plasmid#106013PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)DepositorInsertOpto-GPR27 (GPR27 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
hMEF2A K403R
Plasmid#118355PurposeThis plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.DepositorAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_MLP-sfGFP-CMV_dsRed Exp
Plasmid#117403PurposesfGFP expressed from hypoxia-inducible MLP promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
ExpressionMammalianAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα-mEGFP
Plasmid#114143PurposeMammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
ExpressionMammalianAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP Rab4DN
Plasmid#99019PurposeLocalization and visualization of the dominant negative form of Rab4DepositorInsertRab4 DN (Rab4 Fly, Synthetic)
TagsYFPExpressionMammalianMutationS22N Dominant negativePromoterCMVAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3-Flag-SPRTN-Y117C
Plasmid#110216PurposeExpression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsFlagExpressionMammalianMutationY117C, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Am
Plasmid#109364PurposeHuman rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tagDepositorInsertRod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Branchiostoma belcheri, Human)
Tags1D4ExpressionMammalianPromoterCMVAvailable SinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only