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We narrowed to 18,759 results for: nls

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Showing: 10201 - 10240 of 18759 results

  2. PNLM-pcABE

    Plasmid
    #237618
    Purpose
    PNLM-pcABE generated by pre-trained Protein-Nucleic Acid constrained Language Model exhibits high efficiency, a condensed editing window, and a shortened size.
    Depositor
    Xiaohui Zhang
    Publication
    Ren et al Nat Commun. 2025 Dec 13;16(1):11207. doi
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-8 and 147-152
    Promoter
    pCMV
    Available since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    237618-plasmid-map-sequence-id-495632

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  10. pX461-ABE8e-nSpCas9

    Plasmid
    #237460
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_ABE8e_SpCas9(D10A)_6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237460-plasmid-map-sequence-id-476159

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  11. ICBE

    Plasmid
    #247331
    Purpose
    Vector encoding human codon-optimized engineered DelIscB nickase fusion with APOBEC3A and UGI driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB(D60A)-hAPOBEC3A(W98Y/W104A/Y130F)-2×UGI_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Expression
    Mammalian
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247331-plasmid-map-sequence-id-496500

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  14. pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP

    Plasmid
    #249160
    Purpose
    Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Publication
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-EnAsCas12a-TagBFP (HBB Human, Synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249160-plasmid-map-sequence-id-496435

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  16. pSA097 HNRNPK_IVS3-Cas9-BFP

    Plasmid
    #249147
    Purpose
    Overexpression of SpCas9-BFP with HNRNPK IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Publication
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HNRNPK_IVS3-Cas9-TagBFP (HNRNPK Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HNRNPK IVS3 intron
    Promoter
    EF1a
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249147-plasmid-map-sequence-id-496390

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  18. pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249079
    Purpose
    Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    NNG-Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249079-plasmid-map-sequence-id-496312

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  19. lenti-EF1a-dCas9-VPR-Puro

    Plasmid
    #242162
    Purpose
    Lentiviral expression of dCas9-VPR with puromycin reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64-RelA-Rta; T2A-puro
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242162-plasmid-map-sequence-id-496080

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  26. pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate

    Plasmid
    #238374
    Purpose
    Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes.
    Depositor
    Juan Manuel González-Rosa
    Publication
    Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuclear mKate/3 gRNAs targeting cmlc2
    Promoter
    cmlc2 (nmKate); U6 (gRNAs)
    Available since
    Nov. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    238374-plasmid-map-sequence-id-480246

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  28. snuABE3.1

    Plasmid
    #246011
    Purpose
    snuABE3.1 composed of PhADAR(E438Q) fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
    Depositor
    Sangsu Bae
    Publication
    Im et al bioRxiv 2025.07.19.664718
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q mutant)
    Expression
    Mammalian
    Mutation
    E438Q
    Promoter
    CMV
    Available since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246011-plasmid-map-sequence-id-492301

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  34. pAAV-GFAP-spCas9

    Plasmid
    #231403
    Purpose
    Expresses spCas9 from GFAP promoter
    Depositor
    Arjen Boender, Larry Young
    Insert
    GFAP promoter (Gfap Mouse)
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    GFAP
    Available since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231403-plasmid-map-sequence-id-457979

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  38. L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244187
    Purpose
    PiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Publication
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRed-Express2 under synTF responsive promoter; VEGFR2 NTEVp chain with WT NTEVp; VEGFR1 CTEVp chain; mNeonGreen-P2A-PuroR
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244187-plasmid-map-sequence-id-489486

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  39. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

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  40. pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

    Plasmid
    #242651
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-ABE8.8m-SpCas9-P2A-EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA
    Promoter
    CMV and T7
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242651-plasmid-map-sequence-id-489040

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Showing: 10201 - 10240 of 18759 results