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pSX018 BAC-phagemid CmR(TAG)

Plasmid

#253999

Purpose

BAC-phagemid for LySE, carrying CmR(TAG) for fluctuation analysis. Yeast origin (CEN) and selection (URA3) facilitates assembly in yeast.

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

CmR(TAG)

Expression

Yeast

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pPBG01 Recombination plasmid

Plasmid

#253997

Purpose

Inducible lambdaRED for genomic recombination.

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

lambdaRED

Use

Synthetic Biology

Expression

Bacterial

Available since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-2xEzrin-BioID2-HA

Plasmid

#227681

Purpose

To over express 2x Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Publication

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

2x Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMOD_B-Pho2-1/2-2-tRNA

Plasmid

#161761

Purpose

MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)

Depositor

Shaun Curtin

Publication

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Expression

Bacterial

Promoter

CmYLCV Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV PhiC31 attB dsRed Hygro

Plasmid

#242885

Purpose

Selectable donor plasmid for insertion by PhiC31 integrase into pre-inserted attP landing pad site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Hygromycin-IRE2S-DsRED Express 2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP61

Plasmid

#196799

Purpose

Contains HRM1 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

HYPERSENSITIVE RESPONSE MARKER 1 (AT5G17760 Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_B-Pho2-1/2-2-Csy4

Plasmid

#161760

Purpose

MODULE B Csy4 vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2103 - #91061)

Depositor

Shaun Curtin

Publication

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Expression

Bacterial

Promoter

CmYLCV Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUBI-RZ-Cas12j2

Plasmid

#189781

Purpose

Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-KRasCT

Plasmid

#175836

Purpose

Express iRFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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AiP13927 - pAAV-AiE0140h-minBG-jGCaMP8s-WPRE3-BGHpA

Plasmid

#251749

Purpose

Expression of jGCaMP8s in forebrain Pvalb subclass interneurons

Depositor

Jonathan Ting

Insert

jGCaMP8s

Use

AAV

Available since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b PhiC31 attP41

Plasmid

#242881

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 4a PhiC31 attP41

Plasmid

#242882

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 3b PhiC31 attP41

Plasmid

#242883

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a PhiC31 attP41

Plasmid

#242880

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1P RTEL1_1

Plasmid

#160799

Purpose

Suppress RTEL1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shRTEL1_1

Use

Lentiviral

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25A_2

Plasmid

#160768

Purpose

Suppress CDC25A

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25A_2

Use

Lentiviral

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P RTEL1_2

Plasmid

#160800

Purpose

Suppress RTEL1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shRTEL1_2

Use

Lentiviral

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PRIM2_2

Plasmid

#160796

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_2

Use

Lentiviral

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PRIM2_1

Plasmid

#160795

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_1

Use

Lentiviral

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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