Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Chemically stable fluorescent proteins for advanced microscopy.
Campbell BC, Paez-Segala MG, Looger LL, Petsko GA, Liu CF
Nat Methods. 2022 Nov 7. pii: 10.1038/s41592-022-01660-7. doi: 10.1038/s41592-022-01660-7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
186515Tubulin-hfYFPLocalization of hyperfolder YFP fluorescent protein to tubulin
186518ER2-hfYFPLocalization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
186520pCytERM-hfYFPVisualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)
186522pBAD-hfYFPHyperfolder YFP fluorescent protein: bacterial expression plasmid
186523pcDNA3.1-hfYFPHyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells
186524LifeAct-mhYFPLocalization of monomeric hyperfolder YFP fluorescent protein to actin
186525Tubulin-mhYFPLocalization of monomeric hyperfolder YFP fluorescent protein to tubulin
186526Mito-mhYFPLocalization of monomeric hyperfolder YFP fluorescent protein to mitochondria
186527Clathrin-mhYFPLocalization of monomeric hyperfolder YFP fluorescent protein to clathrin
186528ER2-mhYFPLocalization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
186530pCytERM-mhYFPVisualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)
186532pBAD-mhYFPMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid
186533pcDNA3.1-mhYFPMonomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells
186534LifeAct-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin
186535Tubulin-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin
186536Mito-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria
186537Clathrin-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to clathrin
186538ER2-LSSmGFPLocalization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency
186539H2B-LSSmGFPLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B
186540pCytERM-LSSmGFPVisualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.
186542pBAD-LSSmGFPLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid
186543pcDNA3.1-LSSmGFPLSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells
186544pcDNA3.1-3xhfYFPHyperfolder YFP, triple fusion: cytosolic expression in mammalian cells
186545pcDNA3.1-LSSA12LSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cells
186546pBAD-LSSA12LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid
186547pBAD-FOLD6FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid
186548ER2-mGreenLanternLocalization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency

Antibodies from Article