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pJRL2 PHO5(UASm1)pr VYFP (EB#1549)

Plasmid

#14841

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter UASm1 (PHO5 Budding Yeast)

Tags

VYFP

Expression

Yeast

Mutation

UASm1 (AAGCTT was substituted for CACGTT). Unindu…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)

Plasmid

#14844

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter TATA-C2 (PHO5 Budding Yeast)

Tags

ECFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pT-36

Plasmid

#16252

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

CDC3 (CDC3 Budding Yeast)

Tags

His and T7

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
p0AmpBC

Plasmid

#237275

Purpose

Empty vector for L0 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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p1ChlEF

Plasmid

#237277

Purpose

Empty vector for L1 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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p1ChlFG

Plasmid

#237278

Purpose

Empty vector for L1 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pEB2_17_Q-system-ctrl

Plasmid

#236222

Purpose

Control for Q-system in pEB2_15: Plasmid with mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus.

Depositor

Yvonne Nygård

Article

Blick et al Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf0

Insert

mTurquoise2, ADH1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

5xQUAS_CYC1cp

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pEM110

Plasmid

#250586

Purpose

H2Apr-MRLC-mClover3-FLAG-MRLCter

Depositor

Lillian Fritz-Laylin

Article

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

H2Bpr-P2A-T2A-HygR-Synther8:H2Apr-SpunMLRC-mClover3-SpunMLRCter

Use

Agrobacterium binary plasmid

Tags

mClover3

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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p2KanEG

Plasmid

#237279

Purpose

Empty vector for L2 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pyGAIP-LATeNT-mCherry

Plasmid

#240976

Purpose

Expresses LATeNT-mCherry in yeast cytosol

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

LATeNT-mCherry

Expression

Yeast

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSC-MRscRNA

Plasmid

#240211

Purpose

Yeast vector expressing theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TetO

Expression

Yeast

Promoter

SNR52

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-Pho8∆60-BleMX6

Plasmid

#240974

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; BleMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

BleMX6

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pESC-leu2D-LjCPR1-GW

Plasmid

#233735

Purpose

Expresses plant cytochrome P450 in yeast for enzyme assay. Contains CPR1 from Lotus japonicus

Depositor

Sangeeta Dhaubhadel

Article

Khatri et al PLoS One. 2023 May 15;18(5):e0285698. do

Type

Empty backbone

Expression

Yeast

Promoter

Gal 1 and Gal 10

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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p0AmpCD

Plasmid

#237276

Purpose

Empty vector for L0 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p0AmpAB

Plasmid

#237274

Purpose

Empty vector for L0 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca_Reg1

Plasmid

#237525

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg1

Expression

Yeast

Mutation

Removed binding region 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca

Plasmid

#237524

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Yeast

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGBKT7-AvrPWT4

Plasmid

#237527

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Yeast

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGBKT7-VirPWT4

Plasmid

#237528

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Yeast

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1920

Plasmid

#235767

Purpose

Protein expression of ScVPS34 untagged + ScVPS15 S622D_H623E_T626E-ZZ in yeast

Depositor

Roger Williams

Article

Špokaitė et al eLife

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

S622D, H623E, T626E and ScVPS15 S622D_H623E_T626E

Promoter

GAL-TDH3

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only