We narrowed to 8,312 results for: Dos
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Plasmid#160885PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T1
ExpressionBacterialPromoterrbcS-T1: T7, chaperones: T8Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS5R1AtR2NtB2
Plasmid#160884PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S5
ExpressionBacterialPromoterrbcS-S5: T7, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS2R1AtR2NtB2
Plasmid#160883PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S2, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S2: T7, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)
Plasmid#133790PurposeCAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized NmeCas9
TagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTransgenesis p3 sv40 minimal promoter:: Katushka RFP
Plasmid#131731PurposeTransposon based transgenesisDepositorInsertSV40MinPromoter
UseXenopus expressionAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 L211P
Plasmid#124487Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
TagsHAExpressionMammalianMutationchanged leucine 211 to prolineAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 G381R
Plasmid#124490Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
TagsHAExpressionMammalianMutationchanged glycine at 381 to arginineAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 F416L
Plasmid#124491Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
TagsHAExpressionMammalianMutationchanged phenyalanine 416 to leucineAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.062
Plasmid#119651PurposeLevel 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.DepositorInsertBba_B0011
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V/T230A 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86025PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationLacks mucin-like domain; Changed alanine 82 to va…PromoterCMV IEAvailable SinceJuly 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V/T230A/D637G 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86026PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationLacks mucin-like domain; Changed alanine 82 to va…PromoterCMV IEAvailable SinceJuly 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE
Plasmid#122056PurposeLentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253PDepositorUseLentiviralExpressionMammalianMutationMyoD chimeric protein substituted with the NeuroD…Available SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIDS-RESTmut full-length
Plasmid#109162PurposeEncodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression systemDepositorAvailable SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1-C
Plasmid#109160PurposeEncodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)
ExpressionInsectPromoterp10Available SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC210
Plasmid#118896Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and SNAP tag, cle…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω1
Plasmid#101710PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_LN-HA-AGO2-5xE
Plasmid#92011PurposeExpresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
TagsLambda-N-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V/T230A 2014 EBOV + Mucin-Like Domain
Plasmid#86019PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationChanged alanine 82 to valine and threonine 230 to…PromoterCMV IEAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOG75-Sem1 Sc
Plasmid#83233PurposepCDF-His6-MBP-Sem1 ScDepositorAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only