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JW776

Plasmid

#64817

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

N-terminal domain Firefly

Mutation

rCUC2

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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JW777

Plasmid

#64818

Purpose

BiFC CUC3

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

C-terminal domain Firefly

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pIR197

Plasmid

#64819

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

C-terminal domain Firefly

Mutation

rCUC2

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a+T214V

Plasmid

#66134

Purpose

Expresses the T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Publication

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB1 Cterminal tail

Plasmid

#60400

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB3 Cterminal tail

Plasmid

#60397

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

C127S; TUB2 amino acids 429 - end, replaced with …

Promoter

GAL

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB7 Cterminal tail

Plasmid

#60399

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB8 Cterminal tail

Plasmid

#60401

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB4a Cterminal tail

Plasmid

#60402

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pSALECT-SupH

Plasmid

#59710

Purpose

pSALECT with SupH insert

Depositor

Christopher Anderson

Publication

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

SupH

Expression

Bacterial

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pSALECT-PRAI

Plasmid

#59709

Purpose

pSALECT with PRAI insert

Depositor

Christopher Anderson

Publication

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

PRAI

Expression

Bacterial

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pSALECT-AhyI

Plasmid

#59708

Purpose

pSALECT with AhyI insert

Depositor

Christopher Anderson

Publication

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

AhyI

Expression

Bacterial

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

Plasmid

#111072

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 BD (522-645, C643A)/pET28HMT

Plasmid

#111073

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 BD (522-645, C643A)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.5 AB (361-545, Δ395-511)/pET28HMT

Plasmid

#111267

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.5 AB (361-545, Δ395-511)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

Plasmid

#111398

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

Plasmid

#111399

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

Plasmid

#111400

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

Plasmid

#111451

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-492)/pcDNA3.1

Plasmid

#111454

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-492), C643A

Availability

Academic Institutions and Nonprofits only

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