Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 19,347 results for: EGFP

Clear keywords
Showing: 5361 - 5380 of 19347 results

  2. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    160140-plasmid-map-sequence-id-317331

    Enlarge

  3. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid
    #139992
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139992-plasmid-map-sequence-id-275524

    Enlarge

  4. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

    Plasmid
    #139990
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VQR=D1135V/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139990-plasmid-map-sequence-id-275521

    Enlarge

  5. pAAV_hSyn1_DIO_TVA66T_P2A_EGFP_P2A_N2cG

    Plasmid
    #244253
    Purpose
    AAV plasmid containing hSyn driven DIO expression of TVA66T receptor, EGFP, and CVS rabies N2c glycoprotein
    Depositor
    Tristan Geiller
    Inserts
    TVA receptor 66T
    EGFP
    N2cG
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    244253-plasmid-map-sequence-id-492449

    Enlarge

  6. pHRIG-Akt3

    Plasmid
    #53596
    Purpose
    lentiviral expression of constitutively active human Akt3 and EGFP
    Depositor
    Heng Zhao
    Publication
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    cAkt3 (AKT3 Human)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  8. pCMV-RVR-ABE8e-V106W

    Plasmid
    #193659
    Purpose
    Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-TadA*8e-V106W, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193659-plasmid-map-sequence-id-422134

    Enlarge

  9. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113700
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113700-plasmid-map-sequence-id-231558

    Enlarge

  11. pEGFP-Snapin

    Plasmid
    #118742
    Purpose
    Expresses EGFP tagged Snapin
    Depositor
    Beverly Koller
    Insert
    Snapin cDNA (Snapin Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    118742-plasmid-map-sequence-id-229079

    Enlarge

  17. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid
    #185915
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185915-plasmid-map-sequence-id-367353

    Enlarge

Showing: 5361 - 5380 of 19347 results