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pAAV hSyn:FLEX-Soma-jGCaMP8s

Plasmid

#239669

Purpose

Cre-dependent expression of Soma-jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Publication

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8f

Plasmid

#239671

Purpose

Cre-dependent expression of Soma-jGCaMP8f under hSyn promoter.

Depositor

Marianne Fyhn

Publication

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-SEP

Plasmid

#234541

Purpose

to express a pH sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Publication

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-SEP

Use

AAV

Mutation

None

Promoter

P3

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAav-MDM2-PQS2-3xHA

Plasmid

#84886

Purpose

rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Publication

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

MDM2 genomic sequence HR1 (MDM2 Human)
MDM2 genomic insert HR2 (MDM2 Human)

Use

AAV

Tags

PQS2 3xHA

Promoter

no

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB

Plasmid

#223660

Purpose

Expresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent manner

Depositor

Oscar Marin

Publication

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shLacZ

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-cyto-GCaMP8f

Plasmid

#186050

Purpose

AAV-mediated expression of GCaMP8f under the control of GfaABC1D promoter

Depositor

Loren Looger

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

GfaABC1D

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125234

Purpose

Expresses SPARK oβ2AR transmembrane component in mammalian cells

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40

Plasmid

#124604

Purpose

Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation

Depositor

Christopher Harvey

Publication

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Insert

Soma-localized C1V1(t/t)

Use

AAV

Tags

mRuby2

Expression

Mammalian

Promoter

Ef1a

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-eGFP-2A-mCherry_YDA

Plasmid

#194879

Purpose

Ratiometric YDA sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Publication

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15260 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-3XmiR126TS-BGHpA (Alias: CN5260)

Plasmid

#224194

Purpose

AiE0779m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre(R297T)

Use

AAV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033)

Plasmid

#214852

Purpose

Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has service

AAV PHP.eB

Insert

CoChR-EGFP

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

Plasmid

#184294

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

Depositor

Bernardo Sabatini

Insert

sgRNA-Adrb1

Use

AAV and CRISPR

Expression

Mammalian

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adra1b

Plasmid

#184293

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adra1b

Depositor

Bernardo Sabatini

Inserts

sgRNA-Adra1b
sgRNA-Adra1b

Use

AAV and CRISPR

Expression

Mammalian

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α-DIO-DSE-mCherry-PSE-shRNA-Numb

Plasmid

#250238

Purpose

Cre-dependent AAV expressing mCherry and an shRNA targeting Numb under EF1α promoter for selective Numb knockdown

Depositor

Won Do Heo

Publication

Shin et al Exp Mol Med. 2025 Aug;57(8):1818-1836. d

Insert

Numb (Numb Mouse)

Use

AAV, Cre/Lox, Mouse Targeting, and RNAi

Expression

Mammalian

Promoter

EF1α

Available since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC1-2xmiR-122 target sites

Plasmid

#120302

Purpose

AAV vector for expression of AcrIIC1 with two miR-122 binding sites

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1-2xmiR-122 binding sites

Use

AAV and CRISPR

Expression

Mammalian

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-2xmiR-122 target sites

Plasmid

#120303

Purpose

AAV vector for expression of AcrIIC3 with two miR-122 binding sites

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3-2xmiR-122 binding sites

Use

AAV and CRISPR

Expression

Mammalian

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only
pAAV-EF1α-DIO-cOpn5-T2A-EGFP

Plasmid

#237858

Purpose

The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

Depositor

Minmin Luo

Publication

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV and Cre/Lox

Promoter

EF1α

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGW02 AAV-TBG-Cre-sgP53-sgRNA

Plasmid

#192162

Purpose

AAV-TBG-Cre-sgP53-sgRNA

Depositor

Sidi Chen

Publication

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AiP15285 - pAAV-AiE0170h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5285)

Plasmid

#230509

Purpose

AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13900 - pAAV-AiE0458m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3900)

Plasmid

#224040

Purpose

AiE0458m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons (strong)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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