We narrowed to 11,286 results for: 110

1. B. thetaiotaomicron mRNA toehold switch sensor

   Plasmid

   #110707

   Purpose

   Toehold switch sensor to detect a species specific mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. thetaiotaomicron species specific toehold switch sensor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

   Plasmid

   #110735

   Purpose

   Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   H2B-mOrange2
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)

   Plasmid

   #110145

   Purpose

   Transformation and expression of Csy4 and cogGFP proteins in plants

   Depositor

   Dominique Loque

   Article

   Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

   Insert

   Csy4 (ATCS Mustard Weed)
   

   Use

   Tags

   cogGFP

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p116 Mfz10a

   Plasmid

   #11070

   Depositor

   Ramesh Shivdasani

   Article

   Malik et al Biochem J. 2000 Aug 1;349 Pt 3(Pt 3):829

   Insert

   Mfz10a (Fzd10 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYC6Lys-TRP1URA3

   Plasmid

   #11010

   Depositor

   Francisco Malagon

   Insert

   Pgal1TRP1URA3
   

   Use

   Tags

   V5 and his6

   Expression

   Bacterial and Yeast

   Mutation

   ORF-TRP1 fused to ORF-URA without stop codon

   Promoter

   Available since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-VRERRA-PGK-Puro

   Plasmid

   #110859

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO/Flag-tGFP-Cryz

   Plasmid

   #110378

   Purpose

   Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   crystallin zeta (CRYZ Human)
   

   Use

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p177 Grg-1s EGFP

   Plasmid

   #11066

   Depositor

   Ramesh Shivdasani

   Article

   Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

   Insert

   Grg1-S (Tle1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KG#230

   Plasmid

   #110925

   Purpose

   Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

   Depositor

   Kenneth Miller

   Article

   Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

   Inserts

   unc-129 promoter
   unc-54 3' control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNIC-ZB-SPRTN-Y117C-FL

    Plasmid

    #110219

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    Y117C, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p067 pCS mTRPS1 P4

    Plasmid

    #11039

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    TRPS1 P4 (Trps1 Mouse)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Truncation at aa1121 to mimic frameshift at codon…

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

12. p066 pCS mTRPS1 P3

    Plasmid

    #11038

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    TRPS1 P3 (Trps1 Mouse)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Truncation at aa840 to mimic R840X mutation found…

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

13. p065 pCS mTRPS1 P2

    Plasmid

    #11037

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    TRPS1 P2 (Trps1 Mouse)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Truncation at aa611 to mimic R611X mutation found…

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

14. RON2-COMP-blac-flag-his

    Plasmid

    #110962

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    rhoptry neck protein 2 (RON2) (PF3D7_1452000 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. DENND1A (DENND1AA-c032)

    Plasmid

    #110270

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DENND1A (DENND1A Human)
    

    Use

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSY091

    Plasmid

    #110068

    Purpose

    Bacterial expression of L4

    Depositor

    Jay Keasling

    Article

    Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

    Insert

    L4
    

    Use

    Tags

    Expression

    Mutation

    AT-swapped mutant

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSY075

    Plasmid

    #110067

    Purpose

    Bacterial expression of L2

    Depositor

    Jay Keasling

    Article

    Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

    Insert

    L2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AT-swapped mutant

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-BE3-PGK-Puro

    Plasmid

    #110842

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Hy_pMT dati MCS circfirefly_1677

    Plasmid

    #110118

    Purpose

    Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-VRERRA-P2A-GFP-PGK-Puro

    Plasmid

    #110865

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge