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PL-CRISPR.EFS.tRFP-gIfngr1-2

Plasmid

#166480

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-rxRFP

Plasmid

#67830

Purpose

Redox-Sensitive Red Fluorescent Protein

Depositor

Huiwang Ai

Article

Fan et al Anal Chem. 2015 Mar 3;87(5):2802-10. doi

Insert

Redox-Sensitive Red Fluorescent Protein

Use

Tags

His Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pKeratin-miRFP713-N1

Plasmid

#197235

Purpose

Expresses the fusion protein of Keratin-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Keratin-miRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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Phi3-iRFP

Plasmid

#106296

Purpose

CMV driven iRFP with n terminal membrane binding domain of GAP43

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

iRFPmem

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pActin-miRFP670nano3

Plasmid

#184665

Purpose

Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-human beta-actin

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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iRFP670-KASH2

Plasmid

#187021

Purpose

Inducible disruption of LINC Complex

Depositor

Jan Lammerding

Insert

KASH2

Use

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pmRFP-SUMO1

Plasmid

#13989

Depositor

Jonathan Chernoff

Article

Dadke et al Nat Cell Biol. 2007 Jan . 9(1):80-85.

Insert

SUMO1 (Sumo1 Mouse)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pCytERM-miRFP680-N1

Plasmid

#197183

Purpose

Expresses the fusion protein of CytERM-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-miRFP680

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEB3-miRFP670nano

Plasmid

#127433

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

EB3-miRFP670nano (MAPRE3 Human, Synthetic)

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-(SUMO)3

Plasmid

#127150

Purpose

Mammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Lysosomes-20

Plasmid

#58022

Purpose

Localization: Lysosome Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LAMP1

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
ins:MCS; cryaa:RFP

Plasmid

#110282

Purpose

A zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Tags

Expression

Mutation

Promoter

insulin

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA_RFP-SZ2

Plasmid

#124936

Purpose

Expression of RFP-SZ2

Depositor

Benjamin Schuster, Matthew Good, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Use

Tags

mCherry

Expression

Mammalian

Mutation

SZ2 domain

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-(SIM)1

Plasmid

#127094

Purpose

Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-TrxRFP1

Plasmid

#115327

Purpose

Bacterial expression of TrxRFP1

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP670nano3-NbGFP

Plasmid

#184686

Purpose

Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-NbGFP

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pvimentin-miRFP713

Plasmid

#136569

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-miRFP713

Use

Tags

miRFP713

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p221z-erTagRFP

Plasmid

#71266

Purpose

Entry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

TagRFP

Use

Gateway

Tags

ER retention signal HDEL and signal peptide (ER)…

Expression

Mutation

Promoter

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pVimentin-miRFP703

Plasmid

#79996

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Vimentin (VIM Human)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pNuc-TrxRFP1

Plasmid

#98998

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1 and nuclear localization sequence

Use

Tags

His-tag and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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