We narrowed to 6,084 results for: nls

1. pJL15

   Plasmid

   #174069

   Purpose

   Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

   Depositor

   Albert Keung

   Article

   Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

   Insert

   pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   pGal1

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJF152_Phsp_Cas9

   Plasmid

   #163862

   Purpose

   heat shock-induced expression of Cas9 in C. elegans

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   Cas9, C. elegans codon optimized with intron and SV40_NLS
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Phsp-16.48

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-GFPGO2(NGC)-PGK-Neo

   Plasmid

   #136893

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO2(NGC)
   

   Use

   Lentiviral

   Tags

   2xNLS

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-GFPGO2(NGT)-PGK-Neo

   Plasmid

   #136892

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO2(NGT)
   

   Use

   Lentiviral

   Tags

   2xNLS

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK4a_026

   Plasmid

   #123838

   Purpose

   Encodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   HA::2xNLS::ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK4a_027

   Plasmid

   #123839

   Purpose

   Encodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   HA::2xNLS::GAI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK1_015

   Plasmid

   #123678

   Purpose

   Encodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector LS-2xHS4-LoxP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDA186

   Plasmid

   #74250

   Purpose

   Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).

   Depositor

   Serge Pelet

   Article

   Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

   Insert

   Inducible peptide of the dPSTR
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pGPD1

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDA171

   Plasmid

   #74251

   Purpose

   Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).

   Depositor

   Serge Pelet

   Article

   Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

   Insert

   Inducible peptide of the dPSTR
   

   Use

   Tags

   Venus

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pSTL1

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCP-M-MLV RT∆RNase H

    Plasmid

    #213749

    Purpose

    For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Expression

    Mutation

    H840A

    Promoter

    EF1a

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL-mU6-GFPGO-IRES-mScarletI-PGK-Neo

    Plasmid

    #136897

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    3xNLS on mScarlet

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYN2_1

    Plasmid

    #184757

    Purpose

    Plasmid for genome editing by CRISPR/Cas9

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    

    Use

    Synthetic Biology

    Tags

    2xNLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    PGK1 and tRNA-Phe-HDV Ribozyme

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid

    #84245

    Purpose

    Expresses direct fusion KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    KRAB-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, KRAB, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCH68 (dAsCas12a-3xKRAB piggyBac)

    Plasmid

    #217334

    Purpose

    piggyBac expression of dAsCas12a-3xKRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    dAsCas12a
    

    Use

    CRISPR

    Tags

    nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LbCas12a-niCPE

    Plasmid

    #213747

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-nLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A in LbCas12a

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. MMLV-RT(dRH) for Split-PE (pEH50)

    Plasmid

    #190106

    Purpose

    Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT(delta RNAseH)
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH-mono-MAD7

    Plasmid

    #170134

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    maize Ubiquitin-1, switchgrass Ubiquitin-1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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