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Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins.
Lee J, Mou H, Ibraheim R, Liang SQ, Liu P, Xue W, Sontheimer EJ
RNA. 2019 Aug 22. pii: rna.071704.119. doi: 10.1261/rna.071704.119.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
126202p1146-pCSDest-AcrIIC3Nme-mCherryMammalian expression of AcrIIC3Nme fused to mCherry
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126203p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BSMammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTR
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126204p1148-pCSDest-AcrIIA4-mCherryMammalian expression of AcrIIA4 fused to mCherry
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126205p1149-pCSDest-AcrIIA4-mCherry-miR122BSMammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTR
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129530p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLSSelf-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme
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129531p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BSSelf-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
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129532p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLSSelf-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4
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129534p1195-pssAAV.U1a.hNme2Cas9AAV vector expressing Nme2Cas9
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