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pZS217

Plasmid

#177640

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS221

Plasmid

#177643

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K57A, K58A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS222

Plasmid

#177644

Purpose

In vitro transcription of Patiria miniata Lamp1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Lamp1

Use

In vitro transcription

Tags

3xGFP

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.MCAK-mCherry-eDHFR

Plasmid

#176204

Purpose

MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

MCAK-mCherry-eDHFR

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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3919-32

Plasmid

#176496

Purpose

pCAG-puro-T2A-TetON3G-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

puroR-T2A-TetOn3G

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pZS218

Plasmid

#177641

Purpose

In vitro transcription of Patiria miniata Rab7

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Rab7

Use

In vitro transcription

Tags

mCherry

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pZS230

Plasmid

#177645

Purpose

Bacterial expression of Patiria miniata Dishevelled 1-90

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled 1-90

Tags

GST

Expression

Bacterial

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pZS202

Plasmid

#177638

Purpose

In vitro transcription of Patiria miniata Frizzled 1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Frizzled 1

Use

In vitro transcription

Tags

GFP

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJH806_pJH_LldR_Pcon-sfGFP.1TAG

Plasmid

#163737

Purpose

Constitutive expression of green fluorescent protein encoding one stop codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TAG

Expression

Bacterial

Mutation

gfp.N39TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH28_pBad_AraC_Para-sfGFP.3TAG

Plasmid

#163723

Purpose

Arabinose-inducible expression of green fluorescent protein encoding three amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.3TAG

Expression

Bacterial

Mutation

gfp.N39TAG,N135TAG,Y151TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH626_pJH_LldR_PlldR-sfGFP.1TAG

Plasmid

#163725

Purpose

Lactate-inducible expression of green fluorescent protein encoding one amber codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TAG

Expression

Bacterial

Mutation

gfp.N39TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH627_pJH_LldR_PlldR-sfGFP.2TAG

Plasmid

#163726

Purpose

Lactate-inducible expression of green fluorescent protein encoding two amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TAG

Expression

Bacterial

Mutation

gfp.N39TAG,Y135TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH721_pJH_LldR_PlldR-sfGFP.20TAG

Plasmid

#163729

Purpose

Lactate-inducible expression of green fluorescent protein encoding twenty amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.20TAG

Expression

Bacterial

Mutation

gfp with all 20 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH727_pJH_LldR_PlldR-sfGFP.10TAG

Plasmid

#163731

Purpose

Lactate-inducible expression of green fluorescent protein encoding ten amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH774_pJH_LldR_PlldR-sfGFP.1opal

Plasmid

#163733

Purpose

Lactate-inducible expression of green fluorescent protein encoding one opal codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TGA

Expression

Bacterial

Mutation

gfp with first leucine codon mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 Bighead S-HA

Plasmid

#172485

Purpose

for synthesis of mRNA for injection into xenopus laevis embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

In vitro mrna synthesis

Tags

HA and S-HA

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Tags

HA

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET-11a HIV-1 P6

Plasmid

#154201

Purpose

Bacterial expression of Gag P6 from HIV-1 HXB2 strain

Depositor

Lalit Deshmukh

Article

Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

Insert

Gag P6 protein from HIV-1 clone HXB2

Tags

GB1-6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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