We narrowed to 15,978 results for: grna

1. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

   Plasmid

   #158990

   Purpose

   Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP64
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

   Plasmid

   #233080

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1i

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

   Plasmid

   #165450

   Purpose

   Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9N-IntN
   3x Opn1mw promoter-targeting sgRNAs
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6 and short human rhodopsin promoter

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213969

   Purpose

   AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

   Plasmid

   #233079

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1a

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

   Plasmid

   #230935

   Purpose

   gRNA cloning vector encoding BFP and puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Promoter

   hU6

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

   Plasmid

   #236246

   Purpose

   Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Promoter

   U6 and human Synapsin1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

   Plasmid

   #83919

   Purpose

   Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   DsRed-Express2
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Promoter

   hUbC

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

   Plasmid

   #92121

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-pj23119
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158972

    Purpose

    Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid

    #55201

    Purpose

    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

    Plasmid

    #158988

    Purpose

    Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-KRAB
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid

    #112139

    Purpose

    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

    Inserts

    sgRNA scaffold
    Human-codon-optimized NmeCas9
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U1a and U6

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TBP6.7 non-targeting gRNA lambda phage

    Plasmid

    #132547

    Purpose

    expresses gRNA for TBP-based CIRTS

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    gRNA TBP-based CIRTS
    

    Expression

    Mammalian

    Promoter

    hU6 promoter

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid

    #92120

    Purpose

    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    MS2-P65-HSF1 (HSF1 Synthetic, Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pM123: pAAV-EFS-CasRx-control presgRNA

    Plasmid

    #166871

    Purpose

    AAV vector for expressing CasRx and control presgRNA for RNA-editing

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Inserts

    U6-control presgRNA
    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189924

    Purpose

    AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Mutation

    SaCas9 D10A, TadA ABE8e V106W

    Promoter

    EFS

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

    Plasmid

    #232183

    Purpose

    Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6, hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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