We narrowed to 5,545 results for: donor

1. pHnS 2comp Donor;smFP-V5 KO;Template ORF1

   Plasmid

   #240305

   Purpose

   Donor:smFP-V5 KO:Template

   Depositor

   Yuki Ogawa

   Insert

   Template gRNA
   

   Use

   AAV

   Mutation

   NA

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. KL060 - pBlueScript II SK-mAID-EGFP CTCF donor

   Plasmid

   #232926

   Purpose

   Donor plasmid for tagging mouse CTCF with mAID-eGFP at the N-terminus. Used with KL061 - PX330-sgRNA006_CTCF sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   mAID-eGFP with mCTCF homology arms
   

   Use

   Mouse Targeting

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor

   Plasmid

   #211866

   Purpose

   CENP-T(1-242)-I3-01 transgene integration at AAVS1 safe harbor locus

   Depositor

   Iain Cheeseman

   Insert

   CENP-T(1-242)-I3-01 (CENPT Human, Synthetic)
   

   Use

   CRISPR

   Mutation

   N/A

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199450

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199453

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

   Plasmid

   #199452

   Purpose

   CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)

   Plasmid

   #164047

   Purpose

   CRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA gene

   Depositor

   Baohui Chen

   Article

   Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

   Insert

   mCherry-TriTag
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MADR pDonor smFP-FLAG (dark) WPRE

   Plasmid

   #131005

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-FLAG WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   FLAG - 10 total FLAG tags

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. NanoBRET MLL1/WDR5 Assay Donor Vector, MLL1

   Plasmid

   #237139

   Purpose

   Express MLL1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   MLL1 (KMT2A Human)
   

   Tags

   HaloTag (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

    Plasmid

    #164041

    Purpose

    CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    mCherry-CRISPR-TagV2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

    Plasmid

    #193469

    Purpose

    Cp36 Donor with promoter for mcherry and puro expression

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

    Plasmid

    #85799

    Purpose

    donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Human targeting

    Mutation

    homology arms for knock-in into first intron

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

    Plasmid

    #133560

    Purpose

    Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

    Plasmid

    #172609

    Purpose

    Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    2xFLAG-mAID-AMBRA1 (AMBRA1 Human, Synthetic)
    

    Use

    CRISPR; Human targeting

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid

    #131730

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    TagBFP2-V5 tag

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

    Plasmid

    #164042

    Purpose

    CRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA gene

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    mCherry-CRISPR-TagV2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Desmoplakin II donor only control (FL-based tension sensor)

    Plasmid

    #118723

    Purpose

    The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-FL-mCherry(Y72L)

    Expression

    Mammalian

    Mutation

    inserted FL-based tension sensor module after aa1…

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

    Plasmid

    #221819

    Purpose

    Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    GFP11-HA donor with homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221838

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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