We narrowed to 1,621 results for: nif

1. Lenti-FAB(ER-LD)

   Plasmid

   #214420

   Purpose

   Detection of ER-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and RspAN-FRB-V5

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FAB(PX-LD)

   Plasmid

   #214426

   Purpose

   Detection of peroxisome-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   FKBP-V5
   

   Use

   Tags

   CFAST-Halo and FKBP-V5-RspAN

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-Tagger

   Plasmid

   #129396

   Purpose

   Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

   Insert

   rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
   

   Use

   Tags

   FLAG, HA, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-GG-hUBC-EBFP2

   Plasmid

   #212314

   Purpose

   Lentiviral backbone with EBFP2 reporter

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. DEL-pHG165a

   Plasmid

   #90064

   Purpose

   LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   DEL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LacI-11/109-pHG165c

   Plasmid

   #90059

   Purpose

   Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LacI-11/109
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   109

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGH10 - RlucFluc minus 1 HIV-1

   Plasmid

   #198224

   Purpose

   Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

   Depositor

   Isha Jain

   Article

   Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

   Inserts

   Renilla luciferase
   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET27b-ecDHFR-N23PP/L28F

   Plasmid

   #118584

   Purpose

   Bacterial expression of E.coli DHFR N23PP and L28F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L28 mutated to F28, N23 mutated to P23, a proline…

   Promoter

   T7

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMHE-Pom121-mScarlet

   Plasmid

   #171491

   Purpose

   T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   Pom121 (Pom121 Mouse)
   

   Use

   Pgehme

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMHE-H2B-mClover3

    Plasmid

    #171489

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H2B (H2BC11 Human)
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH727-CEN-RLuc/staCFLuc

    Plasmid

    #38211

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-SLC35B2-sgRNA

    Plasmid

    #154860

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 sgRNA (SLC35B2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #104984

    Purpose

    Expresses ST-Renilla reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LacI/109-pHG165c

    Plasmid

    #90058

    Purpose

    WT LacI polymorphism with T at position 109

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LacI/109
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    109

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-SLC35B2-FLAG

    Plasmid

    #154863

    Purpose

    Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ctcctggtgcagtacttc => ttattagtccaatatttt

    Promoter

    CMV

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH743-CEN-RLuc/slowstaCFLuc

    Plasmid

    #38223

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Halotag-6xHp

    Plasmid

    #214414

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to Halotag

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mApple-1xHp

    Plasmid

    #214409

    Purpose

    Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-RPLP15UTR-hRluc-A60

    Plasmid

    #182639

    Purpose

    Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

    Depositor

    Stephen Floor

    Article

    Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

    Insert

    Renilla luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    The transcription is driven by a T7 promoter, pai…

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEMHE-bNup98-DN-NLS-mClover3

    Plasmid

    #171495

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Nup98-DN (NUP98 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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