We narrowed to 15,965 results for: grna

1. pEG302 5aa SunTag VP64 gRNA4 (FWA)

   Plasmid

   #115481

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG302 5aa SunTag VP64 gRNA17 (FWA)

   Plasmid

   #115482

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL6 sgRNA(MS2)-MS2-hA3a D131E

   Plasmid

   #112132

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a D131E

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a D131E mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D131E mutant in hA3a

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

   Plasmid

   #242660

   Purpose

   CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette

   Depositor

   Benjamin Kleinstiver

   Insert

   pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
   

   Use

   AAV and CRISPR

   Tags

   BPNLS

   Mutation

   VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

   Promoter

   CBh

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYJ29-lenti-U6-SOX17-acti-gRNA-DsRed

   Plasmid

   #131072

   Purpose

   Activation of SOX17 via SAM system

   Depositor

   Xiaojun Lian

   Insert

   SOX17 activation gRNA (SOX17 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYJ30-lenti-U6-MAFA-acti-gRNA1-MS2

   Plasmid

   #131073

   Purpose

   Activation of Mafa via SAM system

   Depositor

   Xiaojun Lian

   Insert

   MAFA activation gRNA1 (MAFA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYJ31-lenti-U6-MAFA-acti-gRNA2-MS2

   Plasmid

   #131074

   Purpose

   Activation of Mafa via SAM system

   Depositor

   Xiaojun Lian

   Insert

   MAFA activation gRNA2 (MAFA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYJ32-lenti-U6-MAFA-acti-gRNA3-MS2

   Plasmid

   #131075

   Purpose

   Activation of Mafa via SAM system

   Depositor

   Xiaojun Lian

   Insert

   MAFA activation gRNA3 (MAFA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYJ33-lenti-U6-Oct4-PE-acti-gRNA-DsRed

   Plasmid

   #131076

   Purpose

   Activation of Mafa via SAM system

   Depositor

   Xiaojun Lian

   Insert

   OCT4 proximal enhancer gRNA (POU5F1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYJ23-lenti-MS2-U6-NKX6.1-acti-gRNA1

    Plasmid

    #131067

    Purpose

    Activation of NKX6.1 via SAM system

    Depositor

    Xiaojun Lian

    Insert

    NKX6.1 activation gRNA1 (NKX6-1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYJ24-lenti-MS2-U6-NKX6.1-acti-gRNA2

    Plasmid

    #131068

    Purpose

    Activation of NKX6.1 via SAM system

    Depositor

    Xiaojun Lian

    Insert

    NKX6.1 activation gRNA2 (NKX6-1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYJ25-lenti-MS2-U6-NKX6.1-acti-gRNA3

    Plasmid

    #131069

    Purpose

    Activation of NKX6.1 via SAM system

    Depositor

    Xiaojun Lian

    Insert

    NKX6.1 activation gRNA3 (NKX6-1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo

    Plasmid

    #239609

    Purpose

    Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    filler-trcr-H1/7SKhybrid-filler
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

    Plasmid

    #240865

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

    Plasmid

    #240094

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid

    #241026

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven empty sgRNA
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid

    #234370

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid

    #234372

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1

    Plasmid

    #220117

    Purpose

    Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    TadA8e, nSauriCas9
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA6

    Plasmid

    #220118

    Purpose

    Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d intron6 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    TadA8e, nSauriCas9
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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