We narrowed to 7,234 results for: CAR

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p362_DG

Plasmid

#234684

Purpose

Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-Opsin

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His6-3C-3XFLAG-SUMO and Thrombin-Opsin

Expression

Bacterial

Mutation

No

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-PH16_RS09790-pcepA-himar-chlorR

Plasmid

#238494

Purpose

Landing Pad with BenM/PH16_RS09790, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Publication

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

BenM/PH16_RS09790

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-PH16_RS08125-pcepA-himar-chlorR

Plasmid

#238493

Purpose

Landing Pad with NahR/PH16_RS08125, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Publication

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

NahR/PH16_RS08125

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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U6-miR.FF4

Plasmid

#235256

Purpose

Non-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FF4 hairpin

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_MSR1

Plasmid

#232391

Purpose

chr8:16099746-16100677 cloned into pGL3

Depositor

Joanna Wysocka

Insert

MSR1 (MSR1 Human)

Use

Luciferase

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

Plasmid

#231356

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-PmSc

Plasmid

#232548

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarlet

Depositor

Calvin Tiengwe

Publication

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Mutation

None

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2ATetP2AP - H

Plasmid

#229972

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2ATetP2AP - H

Use

CRISPR

Expression

Mammalian

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a_HCK

Plasmid

#224239

Purpose

Expression of human HCK Kinase in E. coli

Depositor

Andrew Mesecar

Insert

hck (HCK Human)

Tags

6xHis and 6xHis-TEV

Expression

Bacterial

Promoter

T7 promotor

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-1_Xt-10 kb

Plasmid

#218316

Purpose

Donor plasmid of Tc1-1_Xt Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-3_FR-10 kb

Plasmid

#218317

Purpose

Donor plasmid of Tc1-3_FR Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-1_Xt-5 kb

Plasmid

#218306

Purpose

Donor plasmid of Tc1-1_Xt Transposon with 5 kb cargo gene

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

5 kb cargo gene

Expression

Mammalian

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-3_FR-5 kb

Plasmid

#218307

Purpose

Donor plasmid of Tc1-3_FR Transposon with 5 kb cargo gene

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

5 kb cargo gene

Expression

Mammalian

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR-5 kb

Plasmid

#218308

Purpose

Donor plasmid of Tc1-8B_DR Transposon with 5 kb cargo gene

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

5 kb cargo gene

Expression

Mammalian

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJB48

Plasmid

#226307

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with C to S CsoS2 MR1-6

Expression

Bacterial

Mutation

C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-AGG-NLS-EGFP

Plasmid

#214602

Purpose

express EGFP ; in vitro transcribed modified RNA vector

Depositor

Yuxuan Guo

Publication

Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

Insert

EGFP

Use

Unspecified

Promoter

T7

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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