We narrowed to 8,750 results for: Dos
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Plasmid#160286PurposeAllows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-Bat-CoV-HKU9-3CL
Plasmid#160284PurposeAllows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-TEV-mCherry
Plasmid#105812PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-mCherryExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mRuby3-TEV
Plasmid#105780PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsmRuby3-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mRuby3
Plasmid#105797PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mRuby3ExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo
Plasmid#78353PurposeExpresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)
UseSynthetic BiologyTagsrTetRExpressionMammalianPromoterpEF alphaAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-TEV-mCherry
Plasmid#105800PurposeConcomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCherryExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTwist-WIV1-CoV
Plasmid#164438PurposeEncodes WIV1-CoV Spike Protein for pseudovirus productionDepositorInsertWIV1-CoV Spike
ExpressionMammalianAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKan cpRBP-FL P53
Plasmid#174504PurposeExpress Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)DepositorInsertP53 gene (TP53 Human)
Tags20 amino acid linker containing HRV3C protease si…ExpressionBacterialPromoterT7Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 (1-119aa)
Plasmid#71862Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, first 119 amino acidsPromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-iGluSnFR.A184V
Plasmid#106181PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1InsertSF-iGluSnFR.A184V
UseAAVMutationGltI: A184VPromoterhSynapsinAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3
Plasmid#106206PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVMutationGltI: S72A + mRuby3 fusionPromoterCAGAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63885PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVPromoterEF1aAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-3CL
Plasmid#160280PurposeAllows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330 Rosa26 AscI LH291Gu1 (LH500)
Plasmid#99628PurposePlasmid used to target the pre-modified Rosa26 allele with Cas9DepositorInsertCas9
UseCRISPRTags3xFLAGAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
33-G-X5-pEF1/V5-His
Plasmid#111605PurposeExpress human 33-G-X5 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
TagsFlag tagExpressionMammalianPromoterEF1aAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5080_pHR_PGK_sfGFP_CoV-F2
Plasmid#155304PurposeSARS-CoV-2 fluorescent reporter 2DepositorUseLentiviralExpressionMammalianPromoterPGKAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE
Plasmid#122056PurposeLentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253PDepositorUseLentiviralExpressionMammalianMutationMyoD chimeric protein substituted with the NeuroD…Available SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.5 shETV4-B
Plasmid#74976PurposeshETV4 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF2-V2-FLAG-IRES-GFP
Plasmid#69049Purposelentiviral expression of IKZF2 variant 2 with FLAG tagDepositorInsertIKZF2 (IKZF2 Human)
UseLentiviralTagsFLAG and IRES-GFPExpressionMammalianMutationsplice variant 2PromoterCAGAvailable SinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only