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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,448 results for: nls

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Showing: 5941 - 5960 of 6448 results

  1. L4041

    Plasmid
    #1622
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, SV40NLS, SV40NLS, SV40NLS, gfpN, I…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1622-plasmid-map-sequence-id-232

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  2. L4054

    Plasmid
    #1632
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, SV40NLS, SV40NLS, SV40NLS, gfpN, I…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1632-plasmid-map-sequence-id-242

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  3. L4453

    Plasmid
    #1655
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    rps5-40k, rps55', SV40NLS, SV40NLS, SV40NLS,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1655-plasmid-map-sequence-id-265

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  4. L4686

    Plasmid
    #1673
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, SV40NLS, SV40NLS, SV40N…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1673-plasmid-map-sequence-id-283

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  5. L4019

    Plasmid
    #1615
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2211, SV40NLS, SV40NLS, SV40NLS, SV40NLS,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1615-plasmid-map-sequence-id-225

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  6. L4020

    Plasmid
    #1616
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    rf8134, IVSA, SV40NLS, SV40NLS, SV40NLS, SV40NLS,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1616-plasmid-map-sequence-id-226

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  7. L4037

    Plasmid
    #1619
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, SV40NLS, SV40NLS, SV40N…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1619-plasmid-map-sequence-id-229

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  8. L4042

    Plasmid
    #1623
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hsp16-25', hsp16-415', IVSA, SV40NLS, S…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1623-plasmid-map-sequence-id-233

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  9. L4687

    Plasmid
    #1674
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, SV40NLS, SV40NLS, SV40N…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1674-plasmid-map-sequence-id-284

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  10. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid
    #223123
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9=D10A
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223123-plasmid-map-sequence-id-447704

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  13. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid
    #223114
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223114-plasmid-map-sequence-id-447638

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  14. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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  16. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid
    #197506
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197506-plasmid-map-sequence-id-386645

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  17. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid
    #197505
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…
    Promoter
    CMV and T7
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197505-plasmid-map-sequence-id-386783

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  18. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid
    #188901
    Purpose
    dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    SID-dCas9-XTEN80-KRAB(Kox1)
    Use
    Lentiviral
    Tags
    HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…
    Promoter
    SFFV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188901-plasmid-map-sequence-id-374829

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  19. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid
    #223079
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223079-plasmid-map-sequence-id-447658

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Showing: 5941 - 5960 of 6448 results