We narrowed to 10,132 results for: yeast

1. pEasyG1_nat

   Plasmid

   #184911

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. prp8-G736E

   Plasmid

   #108450

   Purpose

   prp8-G736E in pRS313 backbone for expression in yeast

   Depositor

   Christine Guthrie

   Article

   Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   changed G736 to E

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BAS285 C terminal mKO2 hygBR

   Plasmid

   #74080

   Purpose

   S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   mKO2
   

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pV1539

   Plasmid

   #111434

   Purpose

   Genedrive entry vector for Candida albicans

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCT

   Plasmid

   #60620

   Purpose

   Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDblet

   Plasmid

   #8848

   Depositor

   Joel Huberman

   Article

   Brun et al Gene. 1995 Oct 16. 164(1):173-7.

   Insert

   ARS doublet
   

   Use

   Yeast cloning vector

   Expression

   Yeast

   Mutation

   This vector contains, between AatII sites, a dire…

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. BAS288 C terminal mKO2 natR

   Plasmid

   #74083

   Purpose

   S.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   mKO2
   

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pV1464

   Plasmid

   #111440

   Purpose

   N. castellii Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJZC583

   Plasmid

   #62314

   Purpose

   sgRNA with 2x MS2 for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 2x MS2 binding module
   

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Amida

    Plasmid

    #15362

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    TCF3 (E2A) fusion partner (TFPT Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pJT43_GalL_BE-P(AP)3

    Plasmid

    #145036

    Purpose

    Expresses BE-P(AP)3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-(AP)3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTPI1-EMC1-GFP-APEX2-URA3

    Plasmid

    #184776

    Purpose

    Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    APEX2
    

    Expression

    Yeast

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS415-Cas9-VPR

    Plasmid

    #163971

    Purpose

    Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Insert

    Cas9-VPR
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLLX13

    Plasmid

    #79825

    Purpose

    Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

    Depositor

    Stephen Lory, Cammie Lesser

    Article

    Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Type

    Empty backbone

    Use

    Yeast and bacteria shuttle vector, contains origi…

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. BAS287 C terminal E2C hygBR

    Plasmid

    #74082

    Purpose

    S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    E2Crimson
    

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pRS405 PEX14-VAC8-RFP

    Plasmid

    #166844

    Purpose

    Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, PEX14 Budding Yeast)
    

    Tags

    PEX14 transmembrane domain and RFP

    Expression

    Yeast

    Mutation

    PEX14 transmembrane domain

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-neo-Myc-hApg12

    Plasmid

    #22951

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

    Insert

    autophagy related genes 12 (ATG12 Human)
    

    Tags

    3xMyc

    Expression

    Mammalian

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pCfB1975 (pUC19-PTEF1-PPGK1)

    Plasmid

    #73296

    Purpose

    Example plasmid containing a bidirectional promoter

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    pTEF1-pPGK1
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJZC593

    Plasmid

    #62319

    Purpose

    sgRNA with MS2-PP7 for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + MS2-PP7 RNA binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJZC572

    Plasmid

    #62318

    Purpose

    sgRNA with 1x com for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x com RNA binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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