We narrowed to 7,234 results for: CAR

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pGEX4T-1 PDZD8-CT

Plasmid

#227854

Purpose

To express in E. coli PDZD8-CT

Depositor

Sheng-cai Lin

Publication

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

PDZD8 (PDZD8 Human)

Tags

GST

Expression

Bacterial

Available since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' UBN2

Plasmid

#236678

Purpose

5' AAVLINK plasmid for UBN2 expression

Depositor

Zhonghua Lu

Publication

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

UBN2 (UBN2 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pscAAV.T7-SP6-BC(p11-14)-CAG-EGFP-SV40pA-T7

Plasmid

#231350

Purpose

Self complementary AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

Plasmid

#231353

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

Plasmid

#231357

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

Plasmid

#231358

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

Plasmid

#231359

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7

Plasmid

#231348

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Francisella tularensis enoyl reductase

Plasmid

#231104

Purpose

Expresses FabI under T7 with HIS tag

Depositor

Josh Beckham

Insert

Enoyl-acyl carrier protein reductase

Tags

6x HIS, GST

Expression

Bacterial

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-His-CD86

Plasmid

#232666

Purpose

transient expresses CD86 in mammalian cells

Depositor

Chen-Yang Shen

Publication

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD86 (CD86 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE mTFP1-actin

Plasmid

#229970

Purpose

encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Insert

mTFP1-actin

Use

CRISPR

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE GTS-mTagBFP

Plasmid

#229969

Purpose

encodes a mTagBFP targeted to Golgi apparatus under the control of a dox inducible promoter

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Insert

GTS-mTagBFP

Use

CRISPR

Expression

Mammalian

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE EYFP-tubulin

Plasmid

#229967

Purpose

encodes a YFP-tubulin fusion contruct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Insert

EYFP-tubulin

Use

CRISPR

Expression

Mammalian

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL2368 MTK PB puro backbone

Plasmid

#226673

Purpose

Empty MTK PB cargo backbone marked with puromycin resistance.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2367 MTK PB empty backbone with no marker

Plasmid

#226675

Purpose

Empty MTK PB cargo backbone with no marker.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET50delCT_Vipp1_dL10Ala

Plasmid

#223817

Purpose

Bacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site

Depositor

Carsten Sachse

Publication

Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

Insert

Vipp1

Tags

6xHis

Expression

Bacterial

Mutation

replacement of 10 aa in Hinge 2 with Ala

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

W208L

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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