We narrowed to 9,167 results for: Pol

1. SUMO-Twinkle 43-372

   Plasmid

   #205054

   Purpose

   TWNK protein expression

   Depositor

   Smita Patel

   Article

   Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

   Insert

   TWNK (TWNK Human)
   

   Expression

   Bacterial

   Mutation

   Lacks Mitochondrial localization signal and C ter…

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-HA_aa 321-843 REV1

   Plasmid

   #201587

   Purpose

   Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA repair protein REV1 (REV1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 321-843 of DNA repair prote…

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

   Plasmid

   #201545

   Purpose

   Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks N-terminal residues 1-79

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

   Plasmid

   #201546

   Purpose

   Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 188-228

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

   Plasmid

   #201548

   Purpose

   Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks C-terminal residues 364-409

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBactin-AcGFP-CI

   Plasmid

   #196849

   Purpose

   Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET19-TdTdelta5cys

   Plasmid

   #207465

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTdelta5cys (Dntt Mouse)
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-3UTR

   Plasmid

   #202550

   Purpose

   Ready for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation media

   Depositor

   Rostislav Skrabana

   Article

   Meskova et al Antibodies (Basel). 2023 Aug 4;12(3):51.

   Type

   Empty backbone

   Tags

   ATG initiation codon followed by an IGKV3 signal …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

   Plasmid

   #196877

   Purpose

   Neuron-specific expression of mOrange2 reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBactin-td-mCherry-CI

    Plasmid

    #196851

    Purpose

    Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBetaActin-KIF1aMotor-mScarlet-FKBP

    Plasmid

    #191336

    Purpose

    Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 1-405

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEE005

    Plasmid

    #176762

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    101
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBetaActin-FKBP-mScarlet-KIFC1motorN593K

    Plasmid

    #191335

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1N593K (KIFC1 Human)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEE070

    Plasmid

    #176827

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    709
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEE071

    Plasmid

    #176828

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    710
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEE072

    Plasmid

    #176829

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    711
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEE073

    Plasmid

    #176830

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    712
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEE074

    Plasmid

    #176831

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    713
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEE076

    Plasmid

    #176833

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    715
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEE078

    Plasmid

    #176835

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    717
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge