We narrowed to 9,579 results for: Pol

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peGFP C3-SPG20-PAAA

Plasmid

#218581

Purpose

Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed Proline 172 to Alanine and Tyrosine 174 t…

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-CCR1

Plasmid

#222311

Purpose

Synchronize the trafficking of CCR1 from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLVU-coHPDL WT

Plasmid

#174128

Purpose

Expresses wild type codon-optimized HPDL in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-H1-shScramble-hsynapsin-EGFP

Plasmid

#220795

Purpose

control shRNA plasmid with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT RAD23A

Plasmid

#201458

Purpose

Expresses human RAD23A with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCA13-pHR-SFFV-HaloTag-FBL

Plasmid

#199444

Purpose

Expression of HaloTag-FBL in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

FBL (FBL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRMC2-sec:msfGFP

Plasmid

#194914

Purpose

Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Expression

Bacterial

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQHA-USP7 CS puroR

Plasmid

#46754

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBactin-mNeonGreen-CI

Plasmid

#196850

Purpose

Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAaePUb::Cas9-2A-Neo

Plasmid

#176680

Purpose

Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)

Use

Crispr

Expression

Insect

Mutation

Drosophila codon optimized

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-NQO1

Plasmid

#177140

Purpose

Bacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

NQO1 (NQO1 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-Crx-IRES-GFPd2

Plasmid

#73997

Purpose

Plasmid expressing Crx-IRES- GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Crx-IRES-GFPd2 (Crx Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1995

Plasmid

#49125

Purpose

AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple94-iCre

Use

AAV

Expression

Mammalian

Promoter

GPR88

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV N-term HA-FLAG puro BRD7

Plasmid

#41934

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

HA-Flag

Expression

Mammalian

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pBactin-Tubg1-mEGFP

Plasmid

#196870

Purpose

Expression of the gamma-tubulin fused to enhanced (E)-GFP

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mEGFP (Tubg1 Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mH33B-mClover3

Plasmid

#171496

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

H3.3B (H3f3b Mouse)

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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LLP453 pEf1a-NLS-aGCN4-mCherry

Plasmid

#100945

Purpose

mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, mCherry

Tags

3xTy1

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEE075

Plasmid

#176832

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

714

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM5-Flag

Plasmid

#180410

Purpose

lentiviral transduction of human TGM5 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM5 (TGM5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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