We narrowed to 15,952 results for: grna

1. pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170812

   Purpose

   Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139096

   Purpose

   Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9 3.7
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

   Plasmid

   #113701

   Purpose

   Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

   Plasmid

   #68357

   Purpose

   The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
   FlpO recombinase
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Promoter

   U6 and synthetic Probasin ARRx2

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

   Plasmid

   #113699

   Purpose

   Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176459

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

   Plasmid

   #113700

   Purpose

   Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176460

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

   Plasmid

   #113698

   Purpose

   Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid

    #159676

    Purpose

    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid

    #159677

    Purpose

    Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFG018

    Plasmid

    #62817

    Purpose

    Bacterial inducible gRNA expression plasmid

    Depositor

    Sébastien Rodrigue

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFG001

    Plasmid

    #62816

    Purpose

    Bacterial constitutive gRNA expression plasmid

    Depositor

    Sébastien Rodrigue

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    PN25

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-H1-Esp3I-mU6-Kan

    Plasmid

    #213015

    Purpose

    Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)

    Depositor

    Stephanie Roessler

    Insert

    H1 promoter, mU6 promoter, gRNA scaffolds
    

    Promoter

    H1 promoter

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC-tRNAGln-BbsI-sU6-Kan

    Plasmid

    #213016

    Purpose

    Vector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)

    Depositor

    Stephanie Roessler

    Insert

    tRNAGln promoter, sU6 promoter, gRNA scaffolds
    

    Promoter

    tRNAGln promoter

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pALuWBB

    Plasmid

    #183202

    Purpose

    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

    Depositor

    Mitch McVey

    Insert

    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    

    Use

    CRISPR; Recombines at attp landing site in fly ge…

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide-10X-CS1-3'-puro

    Plasmid

    #212011

    Purpose

    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

    Depositor

    Danwei Huangfu

    Insert

    gRNA scaffold
    

    Use

    Lentiviral

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCJ1023

    Plasmid

    #228763

    Purpose

    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHnS KI;Pdha1 Donor;3xV5 KO;Mff

    Plasmid

    #240301

    Purpose

    KI:Pdha1 Donor:3xV5 KO:MFF

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Pdha1
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHnS KI;Lmnb1 Donor;3xV5 KO;Mecp2

    Plasmid

    #240298

    Purpose

    KI:Lmnb1 Donor:3xV5 KO:Mecp2

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Lmnnb1
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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