We narrowed to 12,966 results for: lic

1. Flag-GFP11-pDEST-N

   Plasmid

   #118366

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   GFP11-Flag

   Expression

   Mammalian

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FlagETR3 (CELF2)

   Plasmid

   #96900

   Purpose

   expression of Flag-tagged human CELF2 (aka CUGBP2)

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   human CELF2 cDNA (CELF2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-AKAR4-NLS

   Plasmid

   #138217

   Purpose

   Nuclear-targeted FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   AKAR4-NLS
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT-ADARcd-E488Q-V5

   Plasmid

   #139686

   Purpose

   Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Adenosine deaminase acting on RNA (Adar Fly)
   

   Tags

   V5

   Expression

   Bacterial and Insect

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-3-FT-ENGRAM

   Plasmid

   #179158

   Purpose

   piggyBac plasmid for 3'-FT ENGRAM recorder

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Insert

   Csy4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   minP

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Thy1 promoter construct

   Plasmid

   #20736

   Depositor

   Joshua Sanes

   Article

   Feng et al Neuron. 2000 Oct . 28(1):41-51.

   Insert

   Thy1 promoter (Thy1 Mouse)
   

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi_RP_3XNLS-MicroID2-FLAG

   Plasmid

   #188523

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   3XNLS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-hPMCA4b

   Plasmid

   #47589

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA4x/b (ATP2B4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K442R and P673L relative to NP_001675.3

   Promoter

   CMV

   Available Since

   Aug. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PZac2.1 hSyn1-BioID2-BioID2-HA

   Plasmid

   #176855

   Purpose

   Expresses the promiscuous small biotin ligase, BioID2, in neurons

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Promoter

   hSyn1

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-HA-GASC1

    Plasmid

    #24214

    Purpose

    Mammalian expression of human JMJD2C with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    GASC1 (KDM4C Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-hGRK4

    Plasmid

    #32690

    Depositor

    Robert Lefkowitz

    Article

    Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

    Insert

    GRK4 (GRK4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5 mSynCAM3

    Plasmid

    #115498

    Purpose

    expression of mouse full-length SynCAM 3

    Depositor

    Thomas Biederer

    Article

    Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

    Insert

    Cadm3 (Cadm3 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-HNF4A-FOXA2-FOXA3

    Plasmid

    #149724

    Purpose

    expressing three liver promoting transcription factors (HNF4A, FOXA2, FOXA3)

    Depositor

    Baisong Lu

    Article

    Lu et al Biomaterials. 2021 Feb;269:120668. doi:

    Insert

    HNF4A-FOXA2-FOXA3
    

    Use

    Lentiviral

    Tags

    P2A, T2A

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-BLU-VIPR

    Plasmid

    #220497

    Purpose

    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBLU-VIPR

    Plasmid

    #220498

    Purpose

    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Expression

    Mammalian

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBbi_RP_2XMTS-MicroID2-FLAG

    Plasmid

    #188524

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    2XMTS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM6-vioABECD

    Plasmid

    #66535

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Consensus T7

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSirt3-RFP

    Plasmid

    #216806

    Purpose

    Human Sirt3-RFP driven by the CaMKII promoter

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

    Insert

    Sirt3 (SIRT3 Human)
    

    Use

    Lentiviral

    Tags

    mRFP1

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Diffusive 41kDa

    Plasmid

    #201335

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-2PrA
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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