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AiP1722 - pAAV-AiE2328m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#242986

Purpose

AiE2328m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Insert

SYFP2

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15641 - pAAV-AiE1419m_3xC3-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#243316

Purpose

AiE1419m_3xC3 is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15245 - pAAV-AiE1058q_3xC2-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#243315

Purpose

AiE1058q_3xC2 is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15708 - pAAV-AiE1129m_3xC1-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#243317

Purpose

AiE1129m_3xC1 is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15692 - pAAV-AiE3242m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#242982

Purpose

AiE3242m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Trygve Bakken, Tanya Daigle, Allen Institute for Brain Science, Boaz Levi, Bosiljka Tasic, Jonathan Ting

Insert

SYFP2

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14676 - pAAV-AiE1270q-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#243311

Purpose

AiE1270q is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Trygve Bakken, Tanya Daigle, Allen Institute for Brain Science, Boaz Levi, Bosiljka Tasic, Jonathan Ting

Insert

SYFP2

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14658 - pAAV-AiE1276q-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#242981

Purpose

AiE1276q is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Trygve Bakken, Tanya Daigle, Allen Institute for Brain Science, Boaz Levi, Bosiljka Tasic, Jonathan Ting

Insert

SYFP2

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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BPK1520-sgRNA GLB1

Plasmid

#184378

Purpose

Expresses a sgRNA to edit GLB1 gene NM_000404.2_c.907A>G

Depositor

David Gilot

Publication

Leclerc et al CRISPR J. 2023 Feb;6(1):17-31. doi: 10.1

Insert

sgRNA targeting GLB1 (GLB1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pOP437

Plasmid

#85936

Purpose

Encrypted AND gate

Depositor

Timothy Lu

Publication

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Encrypted AND gate

Expression

Bacterial

Available since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP447

Plasmid

#85938

Purpose

Encrypted AND gate, decryption step 2.

Depositor

Timothy Lu

Publication

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Encrypted AND gate, decryption step 2

Expression

Bacterial

Available since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-iS-methadoneSnFR-Golgi

Plasmid

#220865

Purpose

Mammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFR

Depositor

Henry Lester

Publication

Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

Insert

iS-methadoneSnFR

Expression

Mammalian

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R82A_pET-14b

Plasmid

#233276

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

Depositor

Mohan Babu, Andrew Emili

Publication

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 82…

Promoter

T7 promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R64A_pET-14b

Plasmid

#233263

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

Depositor

Mohan Babu, Andrew Emili

Publication

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 64…

Promoter

T7 promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#232124

Purpose

A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7CNW)RS

Plasmid

#222874

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLigify_LSorbose

Plasmid

#215562

Purpose

Expresses GFP in response to L-Sorbose

Depositor

Simon d'Oelsnitz, David Ross

Publication

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
sorR

Expression

Bacterial

Promoter

P250 and Psorr

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLigify_Isoeugenol

Plasmid

#215563

Purpose

Expresses GFP in response to Isoeugenol

Depositor

Simon d'Oelsnitz, David Ross

Publication

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
iemR

Expression

Bacterial

Promoter

P250 and Piemr

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLigify_4Vinylphenol

Plasmid

#215564

Purpose

Expresses GFP in response to 4-Vinylphenol

Depositor

Simon d'Oelsnitz, David Ross

Publication

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
vprR

Expression

Bacterial

Promoter

Pvprr

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hIsl2-EGFP

Plasmid

#153206

Purpose

Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Publication

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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ARP3-V5-TurboID

Plasmid

#195144

Purpose

measure COR1C interaction boichemically

Depositor

Gia Voeltz

Publication

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

ACTR3 (ACTR3 Human)

Expression

Mammalian

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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