We narrowed to 62,913 results for: cloning

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SMX DEST vector

Plasmid

#62399

Purpose

Empty Backbone

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Type

Empty backbone

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-B/BRISBANE/60/2008

Plasmid

#187920

Purpose

Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Publication

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB1-B/BRISBANE/60/2008

Plasmid

#187919

Purpose

Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Publication

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Tags

UNTAGGED

Promoter

CMV Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAK-EL-02

Plasmid

#125761

Purpose

Endlinker for tRNA-gRNA system when using only two guides

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-04

Plasmid

#125763

Purpose

Endlinker for tRNA-gRNA system when using only four guides

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX6 (pcDNA3.1+)

Plasmid

#75215

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Publication

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (Slc8b1 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pNCX2

Plasmid

#67148

Purpose

Expresses mRNA of NCX2 (Rat brain)

Depositor

Michela Ottolia, Kenneth Philipson

Publication

Li et al J Biol Chem. 1994 Jul 1;269(26):17434-9.

Insert

NCX2

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

Plasmid

#49313

Purpose

Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

nls-mCherry-Flag

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAGT707

Plasmid

#51835

Purpose

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Tomato Mosaic virus 5'UTR+ CCAT

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41432

Plasmid

#50343

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ocs (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pICSL50007

Plasmid

#50308

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal FLAG tag

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pICSL80005

Plasmid

#50322

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL30003

Plasmid

#50304

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE000

Plasmid

#48860

Purpose

Empty entry vector for creating GreenGate terminator modules.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGGF000

Plasmid

#48861

Purpose

Empty entry vector for creating GreenGate plant resistance cassette modules.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a HAT epitope

Plasmid

#129445

Purpose

expression of HAT epitope

Depositor

Oskar Laur

Insert

HAT epitope

Expression

Bacterial

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-EYFP

Plasmid

#48351

Purpose

Contributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Publication

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EYFP

Use

Gateway Cloning

Mutation

Contains a stop codon.

Promoter

none

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pHBJT023

Plasmid

#225174

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (SmR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT021

Plasmid

#225172

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (GmR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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