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pACT2-Utp15

Plasmid

#99978

Purpose

Express yeast Utp15 with Y2H activation domain fusion

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp15 (UTP15 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Utp15

Plasmid

#99977

Purpose

Express yeast Utp15 with Y2H binding domain fusion

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp15 (UTP15 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
pDONR221-Cka2

Plasmid

#99980

Purpose

Shuttle yeast Cka2 into Gateway destination vector

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka2 (CKA2 Budding Yeast)

Expression

Yeast

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb1

Plasmid

#99981

Purpose

Shuttle yeast Ckb1 into Gateway destination vector

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb1 (CKB1 Budding Yeast)

Expression

Yeast

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb2

Plasmid

#99982

Purpose

Shuttle yeast Ckb2 into Gateway destination vector

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb2 (CKB2 Budding Yeast)

Expression

Yeast

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp11

Plasmid

#99984

Purpose

Shuttle yeast Utp11 into Gateway destination vector

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp11 (UTP11 Budding Yeast)

Expression

Yeast

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp16

Plasmid

#99985

Purpose

Shuttle yeast Utp16 into Gateway destination vector

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp16 (BUD21 Budding Yeast)

Expression

Yeast

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Pfa1

Plasmid

#99999

Purpose

Shuttle yeast Pfa1 into Gateway destination vector

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Pfa1 (SQS1 Budding Yeast)

Expression

Yeast

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Cka1

Plasmid

#99979

Purpose

Shuttle yeast Cka1 into Gateway destination vector

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka1 (CKA1 Budding Yeast)

Expression

Yeast

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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p414CYC_Hsp82_CycTER

Plasmid

#61713

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Publication

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

CYC1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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p414TEF_Hsp82_noTER

Plasmid

#61714

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Publication

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

TEF1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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p414CYC_Hsp82_noTER

Plasmid

#61716

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Publication

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

CYC1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHyg-yeGFP-His6

Plasmid

#212781

Purpose

C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Publication

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pJCHph

Plasmid

#185863

Purpose

The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pETCON-TNF

Plasmid

#67748

Purpose

Tumor Necrosis Factor Alpha Yeast Display Plasmid

Depositor

Timothy Whitehead

Publication

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

TNF-Alpha

Expression

Bacterial and Yeast

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGY466

Plasmid

#166661

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

Depositor

Gaël Yvert

Publication

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

MET17

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFBP-4_1.sensor

Plasmid

#162844

Purpose

Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1

Depositor

Matthias Heinemann

Publication

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

4_1_RNA-device

Expression

Yeast

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFBP-4_1m.mutant

Plasmid

#162869

Purpose

Yeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1

Depositor

Matthias Heinemann

Publication

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

4_1m_RNA-device

Expression

Yeast

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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Gal4pBS12-Pleum

Plasmid

#60326

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

Depositor

Hal Alper

Publication

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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UASGAL-Pleum

Plasmid

#60333

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Publication

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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