We narrowed to 9,174 results for: Pol

1. pX462-hPRKAA2-gRNA_A

   Plasmid

   #74376

   Purpose

   gRNA_A to knockout human AMPK alpha 2 using Cas9n.

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX462-hPRKAA2-gRNA_B

   Plasmid

   #74377

   Purpose

   gRNA_B to knockout human AMPK alpha 2 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS-mEmerald-VIM

   Plasmid

   #62849

   Purpose

   Donor vector for gene editing at human VIM locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   mEmerald-VIM (VIM Human, Synthetic)
   

   Use

   Yeast-e.coli shuttle vector

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-N-Myc_DW164AA USP7

   Plasmid

   #131252

   Purpose

   Mammalian expression of N-terminally Myc-tagged DW164AA USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 aspa…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_6xHis-PARP1-CAT-L713F

   Plasmid

   #173944

   Purpose

   Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTWIN1-His6-Ssp-Httex1-23Q

   Plasmid

   #84349

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFB1.HMBP.PrS.dEZH2

   Plasmid

   #220241

   Purpose

   Expression of an EZH2 catalytic site mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   dEZH2 (EZH2 Human)
   

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   Y731F

   Promoter

   Polyhedrin

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti.PGK.blast-Flag-MFF-WT

   Plasmid

   #74379

   Purpose

   Lentiviral expression vector containing flag tagged WT MFF isoform 5

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pmCherry-N1-EXO1b-D173A

   Plasmid

   #111622

   Purpose

   Expression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cells

   Depositor

   Marco Muzi-Falconi

   Article

   Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

   Insert

   Exonuclease 1b D173A (EXO1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNK115

    Plasmid

    #219745

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal luciferase
    

    Use

    Luciferase and Synthetic Biology

    Mutation

    I3S, N4T, F11L, I63T, T99P, T192S, A199P

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNK116

    Plasmid

    #219742

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Mutation

    D37E, V181I, S323M, M385K

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDH-EF1a-extGluc-NGFR

    Plasmid

    #104832

    Purpose

    extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    extGLuc
    NGFR
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP Cep170 C-term (Nigg GG2)

    Plasmid

    #41151

    Depositor

    Erich Nigg

    Article

    Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

    Insert

    Cep170 (CEP170 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains C-terminal fragment (aa 755-1460, from X…

    Promoter

    CMV, T7

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pFB1.HMBP.PrS.EPOP

    Plasmid

    #125170

    Purpose

    Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    EPOP (EPOP Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mouse b5 full length

    Plasmid

    #217815

    Purpose

    Full length mouse integrin b5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGB5 (Itgb5 Mouse)
    

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCJ207

    Plasmid

    #162680

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTWIN1-His6-Ssp-Httex1-7Q

    Plasmid

    #84347

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal His6-tagged intein Ssp

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28_6xHis-PARP1-L713F

    Plasmid

    #174794

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_6xHis-PARP1-C125G

    Plasmid

    #174792

    Purpose

    Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-C125G (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C125G, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge