We narrowed to 14,132 results for: flag

1. pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

   Plasmid

   #177145

   Purpose

   Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

   Plasmid

   #166814

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1322R

   Promoter

   CMV

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

   Plasmid

   #166811

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   LL1193EE, P1214R, K1277E

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

   Plasmid

   #166812

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H1276G

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

   Plasmid

   #166813

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K1277E

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

   Plasmid

   #128657

   Purpose

   mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L301R/V303R/V306R

   Promoter

   CMV

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJM591 EF1α 3xFLAG-NLS-VP64-ZF1-intN in TUPV2

   Plasmid

   #161567

   Purpose

   Constitutive expression of 3xFLAG-NLS-VP64-ZF1-intN under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-VP64-ZF1-intN
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJM505 EF1α 3xFLAG-NLS-DsRed-Express2-ZF1 in TUPV2

   Plasmid

   #161535

   Purpose

   Constitutive expression of 3xFLAG-NLS-DsRed-Express2-ZF1 under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsRed-Express2-ZF1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJM553 ZF10x6-C 3xFLAG-NLS-DsDed-ZF1-PEST in TUPV2

   Plasmid

   #161540

   Purpose

   Inducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsRed-ZF1-PEST
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   ZF10x6-C

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)

    Plasmid

    #155308

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variant

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfAMA1
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    K546M/I547P/I548Y/I549Y

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)

    Plasmid

    #155313

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    M1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)

    Plasmid

    #155009

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    E1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDAX-017 FLAG Venus173/155[1] IRES Venus173/155[2]

    Plasmid

    #130127

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Venus
    

    Expression

    Mammalian

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid

    #126755

    Purpose

    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

    Plasmid

    #130684

    Purpose

    Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D453_F489del, E653_G1960del

    Promoter

    CMV

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

    Plasmid

    #130683

    Purpose

    Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D409_E452del, E653_G1960del

    Promoter

    CMV

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLU-CMV-Flag-hSesn2 L373A/N376A/D385A/S387A

    Plasmid

    #111789

    Purpose

    Mammalian expression or 3rd generation lentivirus generation of hSesn2

    Depositor

    Jun Hee Lee

    Article

    Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

    Insert

    hSesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L373A/N376A/D385A/S387A

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

    Plasmid

    #70667

    Purpose

    Human mutant of E2F1 lacking amino acids 193-359

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 193 to 359 (E2F1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lacks amino acids 193-359

    Promoter

    cmv

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

    Plasmid

    #70669

    Purpose

    Human mutant of E2F1 lacking amino acids 284 to 359

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 284 to 359 (E2F1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    lacks amin acids 284 to 359

    Promoter

    cmv

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)

    Plasmid

    #50910

    Purpose

    Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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