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We narrowed to 73 results for: flag

Showing: 1 - 20 of 73 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    ...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP...Anti-FLAG frankenbody (FB) construction from the complementarity determining region of an anti-FLAG scFv...targets with nuclear (top, 4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA frankenbodies...here.  Here's what you'll find in this post: Anti-FLAG Frankenbodies for modular imaging of cellular targets...for mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular imaging of cellular targets...Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, modular tool for labeling...

  2. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype version: pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt...you have a Flag tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene If ...insert you would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert ...

  3. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...their antibodies along with some pros and cons. FLAG FLAG is a synthetically derived 8 amino acid tag (DYKDDDDK...tags, FLAG is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag...DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein’s termini to increase...success rates across all applications. You can find FLAG antibodies on Addgene’s catalog including a mouse...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    ...Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags we know and love, the FLAG tag (amino...Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome editing...plan to purify or image a recombinant protein, the FLAG tag has proven itself a good option for essentially... collection includes over 10,000 plasmids with a FLAG tag (!), but did you know that we distribute ready-to-use... (original mouse Fab region with rabbit Fc) anti-FLAG antibody, both of which are also available in trial...

  5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...second plasmid inserts a small epitope tag called FLAG at the end of the transcription factor. It also ... cells with the tagged transcription factor. The FLAG antibody has been well characterized and the system...protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small—20 amino acids—so we don’... selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene: Do you have... flag...

  6. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...we need new epitope tags? Short epitope tags like FLAG, HA, and Myc can adopt a number of different conformations...effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not... observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental effects on...

  7. Plasmids 101: Protein tags

    Type
    Blog Post
    ...protein may be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have ...elution steps use very moderate buffer conditions FLAG DYKDDDD or DYKDDDDK or DYKDDDK 1 Affinity and Purification...friend rather your enemy. Two common tags (SUMO and FLAG) are cleaved by specific proteases without requiring...manner as it would have in the context of a cell. FLAG tags can be cleaved by enterokinase, which recognizes...

  8. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the ... a mammalian selection cassette (e.g. 1435 pSG5L Flag HA). You can also upgrade your basic mammalian expression...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry...

  14. CRISPR Plasmids - Tagging

    Type
    Collection
    ...plasmid FLAG pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR...PX458_AHR_2 ARID4B Human FLAG pFETCh_ARID4B PX458_ARID4B_1 BCL6_iso1 Human FLAG pFETCh_BCL6_iso1 PX458_..._iso1_2 CEBPG Human FLAG pFETCh_CEBPG PX458_CEBPG_1 PX458_CEBPG_2 RAD21 Human FLAG pFETCh_RAD21 PX458_...RAD21 GABPA Human FLAG pFETCh_GABPA PX458_GABPA_1 PX458_GABPA_2 GABPB1_iso2 Human FLAG pFETCh_GABPB1_iso2...PX458_GABPB1_iso2_1 ATF1 Human FLAG pFETCh_ATF1 PX458_ATF1 CEBPA Human FLAG pFETCh_CEBPA PX458_CEBPA_1 PX458..._CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458_TGIF2...TGIF2_2 SSRP1 Human FLAG pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146 PX458...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...8661 p4455 FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS ...HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag CMV Parkinson's... 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon...Arrowsmith 32880 FLAG-MATR3 MATR3 Flag CMV ALS Yossi Shiloh 32881 FLAG-Matr3 delRRM1 MATR3 Flag CMV ALS Yossi...Shiloh 32882 FLAG-Matr3 delRRM2 MATR3 Flag CMV ALS Yossi Shiloh 32883 FLAG-Matr3 delZnF1 MATR3 Flag CMV ALS...Segal 166954 FLAG-KIF5A KIF5A FLAG CMV ALS Rosalind Segal 166955 FLAG-KIF5A delta Tail KIF5A FLAG CMV ALS ...166956 FLAG-KIF5A tail only KIF5A FLAG CMV ALS Rosalind Segal 166957 FLAG-KIF5AR280H KIF5A FLAG CMV ALS...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for ...fusing your protein to an epitope tag, such as Flag or HA, will allow you to easily identify your...expression pNIC-CTHF - C-terminal TEV-His6-Flag for bacterial expression pFA6a vectors - PCR-based...including Xenopus HA Epitope tag pcDNA3.1-HA or c-Flag pcDNA3 - Mammalian expression vector with N- or ... expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression... SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - pDest vector that adds an ... tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG tag in pCS for a variety of...
Showing: 1 - 20 of 73 results