...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP...Anti-FLAG frankenbody (FB) construction from the complementarity determining region of an anti-FLAG scFv...targets with nuclear (top, 4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA frankenbodies...here.  Here's what you'll find in this post: Anti-FLAG Frankenbodies for modular imaging of cellular targets...for mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular imaging of cellular targets...Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, modular tool for labeling...

...pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype version: pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt...you have a Flag tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene If ...insert you would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert ...

...their antibodies along with some pros and cons. FLAG FLAG is a synthetically derived 8 amino acid tag (DYKDDDDK...tags, FLAG is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag...DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein’s termini to increase...success rates across all applications. You can find FLAG antibodies on Addgene’s catalog including a mouse...

...Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags we know and love, the FLAG tag (amino...Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome editing...plan to purify or image a recombinant protein, the FLAG tag has proven itself a good option for essentially... collection includes over 10,000 plasmids with a FLAG tag (!), but did you know that we distribute ready-to-use... (original mouse Fab region with rabbit Fc) anti-FLAG antibody, both of which are also available in trial...

...second plasmid inserts a small epitope tag called FLAG at the end of the transcription factor. It also ... cells with the tagged transcription factor. The FLAG antibody has been well characterized and the system...protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small—20 amino acids—so we don’... selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene: Do you have... flag...

...we need new epitope tags? Short epitope tags like FLAG, HA, and Myc can adopt a number of different conformations...effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not... observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental effects on...

...protein may be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have ...elution steps use very moderate buffer conditions FLAG DYKDDDD or DYKDDDDK or DYKDDDK 1 Affinity and Purification...friend rather your enemy. Two common tags (SUMO and FLAG) are cleaved by specific proteases without requiring...manner as it would have in the context of a cell. FLAG tags can be cleaved by enterokinase, which recognizes...

...our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the ... a mammalian selection cassette (e.g. 1435 pSG5L Flag HA). You can also upgrade your basic mammalian expression...

...Cloning Site (MCS) Adding short tags - ex: HA or Flag Cloning shRNAs Background For the purposes of this...

... on its C-terminus, SpCas9 is also fused to a 3X FLAG tag at its N-terminus. This tag can be used to test...

...different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry...

...with David Goldstein’s lab, will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore the functional...

...plasmid FLAG pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR...PX458_AHR_2 ARID4B Human FLAG pFETCh_ARID4B PX458_ARID4B_1 BCL6_iso1 Human FLAG pFETCh_BCL6_iso1 PX458_..._iso1_2 CEBPG Human FLAG pFETCh_CEBPG PX458_CEBPG_1 PX458_CEBPG_2 RAD21 Human FLAG pFETCh_RAD21 PX458_...RAD21 GABPA Human FLAG pFETCh_GABPA PX458_GABPA_1 PX458_GABPA_2 GABPB1_iso2 Human FLAG pFETCh_GABPB1_iso2...PX458_GABPB1_iso2_1 ATF1 Human FLAG pFETCh_ATF1 PX458_ATF1 CEBPA Human FLAG pFETCh_CEBPA PX458_CEBPA_1 PX458..._CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458_TGIF2...TGIF2_2 SSRP1 Human FLAG pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146 PX458...

...designed for technical writing), it’s great at flagging passive voice, overuse of adverbs, and paragraphs...

...8661 p4455 FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS ...HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag CMV Parkinson's... 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon...Arrowsmith 32880 FLAG-MATR3 MATR3 Flag CMV ALS Yossi Shiloh 32881 FLAG-Matr3 delRRM1 MATR3 Flag CMV ALS Yossi...Shiloh 32882 FLAG-Matr3 delRRM2 MATR3 Flag CMV ALS Yossi Shiloh 32883 FLAG-Matr3 delZnF1 MATR3 Flag CMV ALS...Segal 166954 FLAG-KIF5A KIF5A FLAG CMV ALS Rosalind Segal 166955 FLAG-KIF5A delta Tail KIF5A FLAG CMV ALS ...166956 FLAG-KIF5A tail only KIF5A FLAG CMV ALS Rosalind Segal 166957 FLAG-KIF5AR280H KIF5A FLAG CMV ALS...

...resulted in the development of a new plasmid, pCMV14-3X-Flag-SARS-CoV-2 S, which is now easily available through...

...are available for tagging with epitope tags (e.g. FLAG and STREP) and auxin-inducible degrons (AID). Further...

...with C-term 3X FLAG tag) 43861 JDS246 (mammalian codon-optimized Cas9 with C-term 3X FLAG tag)...endonuclease. Both vectors also express a C-terminal 3X FLAG tag. MLM3639 is the same as the Cas9 expression ...zebrafish (MLM3613) except for the addition of the 3X FLAG tag. JDS246 has been codon optimized for expression...

...Gag-Cas9 fusion protein The MLV Gag protein fused to a Flag-tagged SpCas9 is incorporated into Nanoblades, just...

...finer points of the protocols. Are there any red flags about how the work was performed that could influence...